Overview

  • Product name
    Anti-Connexin 45 / GJA7 antibody
    See all Connexin 45 / GJA7 primary antibodies
  • Description
    Rabbit polyclonal to Connexin 45 / GJA7
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human Connexin 45/ GJA7.

    (Peptide available as ab91444.)

  • Positive control
    • This antibody gave a positive signal in Rat Lung Tissue Lysate and in the following whole cell lysates: SHSY5Y; SKNBE; SKNSH.

Properties

Applications

Our Abpromise guarantee covers the use of ab70365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 45 kDa).

Target

Images

  • All lanes : Anti-Connexin 45 / GJA7 antibody (ab70365) at 1 µg/ml

    Lane 1 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 2 : SK N BE (Human neuroblastoma) Whole Cell Lysate
    Lane 3 : SK N SH (Human neuroblastoma) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 35 kDa,68 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 8 minutes
  • Anti-Connexin 45 / GJA7 antibody (ab70365) at 1 µg/ml + Lung (Rat) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa


    Exposure time : 20 minutes

References

ab70365 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab70365.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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