The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/250 - 1/500.
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Perform Heat Induced Epitope Retrieval (HIER) at pH 6.
FunctionRequired for targeting of connexins to the plasma membrane.
Sequence similaritiesBelongs to the CNST family.
Cellular localizationCell membrane. Golgi apparatus > trans-Golgi network membrane. Cytoplasmic vesicle > secretory vesicle. Located predominantly in the trans-Golgi network. Probably trafficks between the trans-Golgi network and the cell membrane via the secretory pathway.