Anti-Constitutive androstane receptor antibody (ab83850)

Overview

  • Product name
    Anti-Constitutive androstane receptor antibody
    See all Constitutive androstane receptor primary antibodies
  • Description
    Rabbit polyclonal to Constitutive androstane receptor
  • Tested applications
    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Chimpanzee, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 180-229 (PVFRSLPIED QISLLKGAAV EICHIVLNTT FCLQTQNFLC GPLRYTIEDG) of human Constitutive androstane receptor (NP_001070939).

  • Positive control
    • Jurkat cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab83850 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

Target

  • Function
    Binds and transactivates the retinoic acid response elements that control expression of the retinoic acid receptor beta 2 and alcohol dehydrogenase 3 genes. Transactivates both the phenobarbital responsive element module of the human CYP2B6 gene and the CYP3A4 xenobiotic response element.
  • Tissue specificity
    Predominantly expressed in liver.
  • Sequence similarities
    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Domain
    Composed by a short N-terminal domain followed by the DNA binding, hinge, and ligand binding/dimerization domains.
  • Post-translational
    modifications
    Phosphorylated at Thr-38 by PKC, dephosphorylation of Thr-38 is required for nuclear translocation and activation.
  • Cellular localization
    Nucleus. Cytoplasm. Cytoplasm > cytoskeleton. Recruited to the cytoplasm by DNAJC7.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAR antibody
    • CAR BETA antibody
    • CAR1 antibody
    • CARA antibody
    • Constitutive activator of retinoid response antibody
    • constitutive active receptor antibody
    • Constitutive active response antibody
    • constitutive androstane nuclear receptor variant 2 antibody
    • constitutive androstane nuclear receptor variant 3 antibody
    • constitutive androstane nuclear receptor variant 4 antibody
    • constitutive androstane nuclear receptor variant 5 antibody
    • Constitutive androstane receptor antibody
    • MB67 antibody
    • MGC97144 antibody
    • NR1I3 antibody
    • NR1I3_HUMAN antibody
    • Nuclear receptor subfamily 1 group I member 3 antibody
    • orphan nuclear hormone receptor antibody
    • Orphan nuclear receptor MB67 antibody
    • Orphan nuclear receptor NR1I3 antibody
    see all

Images

  • Anti-Constitutive androstane receptor antibody (ab83850) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 31 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue labelling Constitutive androstane receptor with ab83850 at 5.0µg/ml.

References

ab83850 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
ChIP
Sample
Bos taurus Cell lysate - nuclear (BFH12 (bovine foetal hepatocyte-like) cell line)
Negative control
Negative control: only beads. Negative control locus: bovine CYP3A28 (CYP3A4-like) coding sequence (exon 13).
Specification
BFH12 (bovine foetal hepatocyte-like) cell line
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde 1%
Positive control
Positive control antibody: rabbit polyclonal to hystone H3 (AB1791-100). Positive control locus: not known cause of lacking information on CAR-mediated gene regulation in bovine. Promoter regions tested: 1. bovine CYP3A28 proximal promoter containing ER6 binding site, predicted to be specific for PXR 2. a distal region (about - 5000 bp from TSS), located in bovine CYP3A28 promoter, containing DR5 binding site, predicted to be specific for CAR
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Verified customer

Submitted Oct 31 2017

Application
Western blot
Sample
Bos taurus Tissue lysate - nuclear (liver)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
30 µg
Specification
liver
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Verified customer

Submitted Oct 26 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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