Overview

  • Product nameAnti-Contactin 1 antibody [S73-20]
    See all Contactin 1 primary antibodies
  • Description
    Mouse monoclonal [S73-20] to Contactin 1
  • Tested applicationsSuitable for: Flow Cyt, WB, IHC-P, IHC-Fr, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to extracellular amino acids 21 - 37 (

    D/EFTWHRRYGHGVSEEDKC

    ) of Rat Contactin 1 (A57112).

Properties

Applications

Our Abpromise guarantee covers the use of ab105582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 113 kDa.
IHC-P Use a concentration of 0.1 - 1 µg/ml.
IHC-Fr Use a concentration of 0.1 - 1 µg/ml.
ICC Use a concentration of 0.1 - 1 µg/ml.
ICC/IF Use a concentration of 1 - 10 µg/ml.

Target

  • FunctionContactins mediate cell surface interactions during nervous system development. Involved in the formation of paranodal axo-glial junctions in myelinated peripheral nerves and in the signaling between axons and myelinating glial cells via its association with CNTNAP1. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Interaction with TNR induces a repulsion of neurons and an inhibition of neurite outgrowth.
  • Tissue specificityStrongly expressed in brain and in neuroblastoma and retinoblastoma cell lines. Lower levels of expression in lung, pancreas, kidney and skeletal muscle.
  • Involvement in diseaseDefects in CNTN1 are the cause of Compton-North congenital myopathy (CNCM) [MIM:612540]. CNCM is a familial lethal form of congenital onset muscle weakness, inherited in an autosomal-recessive fashion and characterized by a secondary loss of beta2-syntrophin and alpha-dystrobrevin from the muscle sarcolemma, central nervous system involvement, and fetal akinesia.
  • Sequence similaritiesBelongs to the immunoglobulin superfamily. Contactin family.
    Contains 4 fibronectin type-III domains.
    Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CNTN 1 antibody
    • CNTN antibody
    • Cntn1 antibody
    • CNTN1_HUMAN antibody
    • Contactin-1 antibody
    • Contactin1 antibody
    • F3 antibody
    • F3cam antibody
    • Glycoprotein gp135 antibody
    • gp 135 antibody
    • GP135 antibody
    • MYPCN antibody
    • Neural cell surface protein F3 antibody
    see all

Anti-Contactin 1 antibody [S73-20] images

  • Overlay histogram showing SH-SY5Y cells stained with ab105582 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab105582, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed SH-SY5Y cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-Contactin 1 antibody [S73-20] (ab105582)

This product has been referenced in:
  • Rosenbaek LL  et al. Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis. J Biol Chem 289:13347-61 (2014). Read more (PubMed: 24668812) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"