Overview

  • Product name
  • Description
    Rabbit polyclonal to Contactin 6
  • Specificity
    Specificity of this product was demonstrated by immunohistochemistry where neurons were intensely stained in rat dorsal root ganglia. Oligodendrocytes in the rat trigeminal nerve root are also stained.
  • Tested applications
    Suitable for: ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    A peptide corresponding to an antigenic region within the carboxy-terminal domain of the rat Contactin 6 protein. This peptide was conjugated to carrier protein to enhance the immunological response.

Properties

Applications

Our Abpromise guarantee covers the use of ab49207 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IHC-Fr
  • Application notes
    1 site ELISA: 1/5000.
    IHC-Fr: 1/4000.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Contactins mediate cell surface interactions during nervous system development. Participates in oligodendrocytes generation by acting as a ligand of NOTCH1. Its association with NOTCH1 promotes NOTCH1 activation through the released notch intracellular domain (NICD) and subsequent translocation to the nucleus. Involved in motor coordination.
    • Tissue specificity
      Expressed in nervous system. Highly expressed in cerebellum. Expressed at intermediate level in thalamus, subthalamic nucleus. Weakly expressed in corpus callosum, caudate nucleus and spinal cord.
    • Sequence similarities
      Belongs to the immunoglobulin superfamily. Contactin family.
      Contains 4 fibronectin type-III domains.
      Contains 6 Ig-like C2-type (immunoglobulin-like) domains.
    • Cellular localization
      Cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • CNTN 6 antibody
      • CNTN6 antibody
      • CNTN6_HUMAN antibody
      • Contactin 6 precursor antibody
      • Contactin-6 antibody
      • Contactin6 antibody
      • hNB 3 antibody
      • hNB-3 antibody
      • hNB3 antibody
      • MGC133256 antibody
      • NB 3 antibody
      • NB3 antibody
      • Neural adhesion molecule antibody
      • Neural recognition molecule NB 3 antibody
      • Neural recognition molecule NB-3 antibody
      see all

    References

    ab49207 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brain)
    Permeabilization
    Yes - -20 Methanol, 1 min
    Specification
    brain
    Blocking step
    3% BSA, 5%Donkey serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Username

    Dr. Nina Mayorek

    Verified customer

    Submitted Nov 16 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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