Overview

  • Product name
  • Description
    Mouse polyclonal to COP
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-97 of Human COP (NP_443121.1).

  • Positive control
    • COP transfected 293T cell lysate; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab168243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 11 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Caspase inhibitor. Acts as a regulator of procaspase-1/CASP1 activation implicated in the regulation of the proteolytic maturation of pro-interleukin-1 beta (IL1B) and its release during inflammation. Inhibits the release of IL1B in response to LPS in monocytes. Also induces NF-kappa-B activation during the pro-inflammatory cytokine response. Also able to inhibit CASP1-mediated neuronal cell death, TNF-alpha, hypoxia-, UV-, and staurosporine-mediated cell death but not ER stress-mediated cell death. Acts by preventing activation of caspases CASP1 and CASP4, possibly by preventing the interaction between CASP1 and RIPK2.
  • Tissue specificity
    Widely expressed. Expressed at higher level in placenta, spleen, lymph node and bone marrow. Weakly or not expressed in thymus.
  • Sequence similarities
    Contains 1 CARD domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • CAR16_HUMAN antibody
    • CARD only domain-containing protein 1 antibody
    • CARD only protein antibody
    • CARD16 antibody
    • Caspase recruitment domain family, member 16 antibody
    • Caspase recruitment domain-containing protein 16 antibody
    • Caspase-1 dominant-negative inhibitor pseudo-ICE antibody
    • Caspase-1 inhibitor COP antibody
    • COP antibody
    • COP1 antibody
    • Pseudo interleukin 1 beta converting enzyme antibody
    • Pseudo interleukin-1 beta converting enzyme antibody
    • Pseudo interleukin-1beta converting enzyme antibody
    • PSEUDO-ICE antibody
    see all

Images

  • All lanes : Anti-COP antibody (ab168243) at 1 µg/ml

    Lane 1 : COP transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Developed using the ECL technique.

    Predicted band size: 11 kDa

  • Immunofluorescent analysis of permeabilized HeLa cells labeling COP with ab168243 at 10µg/ml.

References

ab168243 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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