Overview

  • Product nameAnti-COP1 antibody
    See all COP1 primary antibodies
  • Description
    Rabbit polyclonal to COP1
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Human, Pig
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 301-350 (MSGLYSPVSE DSTVPQFEAP SPSHSSIIDS TEYSQPPGFS GTSQTKKQPW) of Mouse COP1 (NP_036061).

  • Positive control
    • Mouse thymus lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab116122 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 80 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionE3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.
  • Tissue specificityUbiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.
  • PathwayProtein modification; protein ubiquitination.
  • Sequence similaritiesBelongs to the COP1 family.
    Contains 1 RING-type zinc finger.
    Contains 7 WD repeats.
  • DomainThe RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS).
  • Cellular localizationNucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Constitutive photomorphogenesis protein 1 homolog antibody
    • COP antibody
    • E3 ubiquitin-protein ligase RFWD2 antibody
    • hCOP1 antibody
    • Rfwd2 antibody
    • RFWD2_HUMAN antibody
    • ring finger and WD repeat domain 2 antibody
    • RING finger and WD repeat domain protein 2 antibody
    • RING finger protein 200 antibody
    see all

Anti-COP1 antibody images

  • Anti-COP1 antibody (ab116122) at 1 µg/ml + Mouse thymus lysate at 10 µg

    Predicted band size : 80 kDa

References for Anti-COP1 antibody (ab116122)

ab116122 has not yet been referenced specifically in any publications.

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