Overview

  • Product name
  • Description
    Rabbit polyclonal to COP1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    A region between residue 1 and 50 of human COP1. (NP_071902.2)

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab70889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IP Use a concentration of 2 - 5 µg/ml.

Target

  • Function
    E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.
  • Tissue specificity
    Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.
  • Pathway
    Protein modification; protein ubiquitination.
  • Sequence similarities
    Belongs to the COP1 family.
    Contains 1 RING-type zinc finger.
    Contains 7 WD repeats.
  • Domain
    The RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS).
  • Cellular localization
    Nucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles.
  • Information by UniProt
  • Database links
  • Alternative names
    • Constitutive photomorphogenesis protein 1 homolog antibody
    • COP antibody
    • E3 ubiquitin-protein ligase RFWD2 antibody
    • hCOP1 antibody
    • Rfwd2 antibody
    • RFWD2_HUMAN antibody
    • ring finger and WD repeat domain 2 antibody
    • RING finger and WD repeat domain protein 2 antibody
    • RING finger protein 200 antibody
    see all

Images

  • All lanes : Anti-COP1 antibody (ab70889) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 80 kDa
    Observed band size : 80 kDa


    Exposure time : 3 minutes
  • HeLa whole cell lysate (at 1 mg for IP, 20% of IP loaded), with ab70889 at 1 µg/ml for WB, subsequent to IP at 3 µg/mg lysate.
    Detection: Chemiluminescence with an exposure time of 10 seconds.

References

ab70889 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting Abcam.

The antibody ab70889, should recognize both overexpressed and endogenous forms of the protein. As for negative controls, our notes indicate that the protein is ubiquitously expressed at low level and expressed ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up