Anti-COP1 antibody (ab70890)
Key features and details
- Rabbit polyclonal to COP1
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-COP1 antibody
See all COP1 primary antibodies -
Description
Rabbit polyclonal to COP1 -
Host species
Rabbit -
Tested applications
Suitable for: IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Pig, Chimpanzee, Rhesus monkey, Orangutan -
Immunogen
A region between residues 100 and 150 of human COP1. (NP_071902.2)
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Positive control
- HeLa whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70890 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP |
Use a concentration of 2 - 5 µg/ml.
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Notes |
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IP
Use a concentration of 2 - 5 µg/ml. |
Target
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Function
E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. -
Tissue specificity
Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Belongs to the COP1 family.
Contains 1 RING-type zinc finger.
Contains 7 WD repeats. -
Domain
The RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS). -
Cellular localization
Nucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles. - Information by UniProt
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Database links
- Entrez Gene: 64326 Human
- Omim: 608067 Human
- SwissProt: Q8NHY2 Human
- Unigene: 523744 Human
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Alternative names
- Constitutive photomorphogenesis protein 1 homolog antibody
- COP antibody
- E3 ubiquitin-protein ligase RFWD2 antibody
see all
Images
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HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
Antibodies:
Lane 1: ab70889 at 3µg/ml for IP.
Lane 2: ab70890 at 3µg/ml for IP.
Lane 3: ab70891 at 3µg/ml for IP.
Lane 4: IgG control
Subsequent Western Blotting was carried out using ab70889 at 1µg/ml.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab70890 has not yet been referenced specifically in any publications.