Overview

  • Product name
  • Description
    Rabbit polyclonal to COP1
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    A region between residues 100 and 150 of human COP1. (NP_071902.2)

  • Positive control
    • HeLa whole cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab70890 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
  • Application notes
    IP: Use at a concentration of 2 - 5 µg/ml.


    Is unsuitable for WB. (Use ab70889).



    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.
    • Tissue specificity
      Ubiquitously expressed at low level. Expressed at higher level in testis, placenta, skeletal muscle and heart.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Belongs to the COP1 family.
      Contains 1 RING-type zinc finger.
      Contains 7 WD repeats.
    • Domain
      The RING finger domain, in addition to its role in ubiquitination, functions as a structural scaffold to bring two clusters of positive-charged residues within spatial proximity to mimic a bipartite nuclear localization signal (NLS).
    • Cellular localization
      Nucleus speckle. Cytoplasm. In the nucleus, it forms nuclear speckles.
    • Information by UniProt
    • Database links
    • Alternative names
      • Constitutive photomorphogenesis protein 1 homolog antibody
      • COP antibody
      • E3 ubiquitin-protein ligase RFWD2 antibody
      • hCOP1 antibody
      • Rfwd2 antibody
      • RFWD2_HUMAN antibody
      • ring finger and WD repeat domain 2 antibody
      • RING finger and WD repeat domain protein 2 antibody
      • RING finger protein 200 antibody
      see all

    Images

    • HeLa whole cell lysate (1 mg for IP, 20% of IP loaded).
      Antibodies:
      Lane 1: ab70889 at 3µg/ml for IP.
      Lane 2: ab70890 at 3µg/ml for IP.
      Lane 3: ab70891 at 3µg/ml for IP.
      Lane 4: IgG control
      Subsequent Western Blotting was carried out using ab70889 at 1µg/ml.
      Detection: Chemiluminescence with an exposure time of 10 seconds.

    References

    ab70890 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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