- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-COPRS antibody
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DescriptionRabbit polyclonal to COPRS
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment corresponding to Human COPRS aa 33-106.
Sequence:EAGFATADHSGQERETEKAMDRLARGTQSIPNDSPARGEGTHSEEEGFAM DEEDSDGELNTWELSEGTNCPPKE
Database link: Q9NQ92 -
Positive control
- Human liver tissue lysate; Human cell line MCF-7; Human lymph node tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab185467 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/250 - 1/500. Predicted molecular weight: 20 kDa. | |
| IHC-P | 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | Use a concentration of 1 - 4 µg/ml. |
Target
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FunctionHistone-binding protein required for histone H4 methyltransferase activity of PRMT5. Specifically required for histone H4 'Arg-3' methylation mediated by PRMT5, but not histone H3 'Arg-8' methylation, suggesting that it modulates the substrate specificity of PRMT5. Specifically interacts with the N-terminus of histone H4 but not with histone H3, suggesting that it acts by promoting the association between histone H4 and PRMT5. Involved in CCNE1 promoter repression. Plays a role in muscle cell differentiation by modulating the recruitment of PRMT5 to the promoter of genes involved in the coordination between cell cycle exit and muscle differentiation.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 55352 Human
- SwissProt: Q9NQ92 Human
- Unigene: 462729 Human
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Alternative names
- C17orf79 antibody
- Cooperator of PRMT5 antibody
- Coordinator of PRMT5 and differentiation stimulator antibody
see all
Images
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Western blot - Anti-COPRS antibody (ab185467)All lanes : Anti-COPRS antibody (ab185467) at 1/250 dilution
Lane 1 : Human RT-4 lysate
Lane 2 : Human U-251MG lysate
Lane 3 : Human plasma (IgG/HSA depleted) lysate
Lane 4 : Human liver tissue lysate
Predicted band size: 20 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-COPRS antibody (ab185467)Immunofluorescent analysis of PFA/Triton X-100 fixed Human cell line MCF-7, labeling COPRS with ab185467 at 4µg/ml.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COPRS antibody (ab185467)Immunohistochemical staining of formalin fixed paraffin-embedded Human lymph node tissue labeling COPRS with ab185467 at 1/200 dilution.
Protocols
References
ab185467 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"