Human Predicted to work with:
Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Xenopus laevis, Chimpanzee, Zebrafish, Rhesus monkey, Orangutan, Platypus (Ornithorhynchus anatinus)
Immunogen was a synthetic peptide, which represented a portion of human COP9 signalosome complex subunit 3 encoded within exon 10 (LocusLink ID 8533).
Whole cell lysate from mock transfected or CSN3 transfected HEK293T cells.
CSN3 is a subunit of the JUN activation-domain binding protein 1 (JAB1), containing signalosome complex (or COP9 complex) which has kinase activity and phosphorylates C-JUN. The signalosome complex may play a role in signal transduction.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Purification notesAntibodies were affinity purified using the peptide immobilized on solid support.
Primary antibody notesCSN3 is a subunit of the JUN activation-domain binding protein 1 (JAB1), containing signalosome complex (or COP9 complex) which has kinase activity and phosphorylates C-JUN. The signalosome complex may play a role in signal transduction.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/20000. Detects a band of approximately 37 kDa (predicted molecular weight: 51 kDa).
FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
Tissue specificityWidely expressed. Expressed at high level in heart and skeletal muscle.
Sequence similaritiesBelongs to the CSN3 family. Contains 1 PCI domain.
ab10463 detection of human CSN3 by Western blot. Samples: Whole cell lysate (E = 50 µg; T = 10 µg) from mock transfected (E) or CSN3 transfected (T) HEK293T cells. Antibody: ab10463 used at the indicated concentration. Detection: 10 second exposure with chemiluminescence.
References for Anti-COPS3 antibody (ab10463)
has not yet been referenced specifically in any publications.
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