Overview

  • Product nameAnti-COPS3 antibody
    See all COPS3 primary antibodies
  • Description
    Goat polyclonal to COPS3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Xenopus laevis, Zebrafish, Cynomolgus Monkey, Xenopus tropicalis
  • Immunogen

    Synthetic peptide:

    C-SLYKKNIQRLT

    , corresponding to internal sequence amino acids 301-311 of Human COPS3 (NP_003644.2).

  • Positive control
    • HEK293 lysate overexpressing Human COPS3 with DYKDDDDK tag.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferpH: 7.30
    Preservative: 0.02% Sodium azide
    Constituents: 99% Tris buffered saline, 0.5% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab113365 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab113365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 48 kDa).

Target

  • FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
  • Tissue specificityWidely expressed. Expressed at high level in heart and skeletal muscle.
  • Sequence similaritiesBelongs to the CSN3 family.
    Contains 1 PCI domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Constitutive photomorphogenic homolog subunit 3 antibody
    • COP 9 (constitutive photomorphogenic) subunit 3 antibody
    • COP 9 complex homolog subunit 3 antibody
    • COP 9 complex S3 antibody
    • COP 9 complex subunit 3 antibody
    • COP 9 constitutive photomorphogenic homolog subunit 3 antibody
    • COP 9 homolog antibody
    • COP 9 signalosome complex subunit 3 antibody
    • COP 9 signalosome subunit 3 antibody
    • COP 9 subunit 3 antibody
    • COP9 (constitutive photomorphogenic) subunit 3 antibody
    • COP9 complex homolog subunit 3 antibody
    • COP9 complex S3 antibody
    • COP9 complex subunit 3 antibody
    • COP9 constitutive photomorphogenic homolog subunit 3 antibody
    • COP9 homolog antibody
    • COP9 signalosome complex subunit 3 antibody
    • COP9 signalosome subunit 3 antibody
    • COP9 subunit 3 antibody
    • COPS 3 antibody
    • cops3 antibody
    • CSN 3 antibody
    • CSN3 antibody
    • CSN3_HUMAN antibody
    • JAB 1 containing signalosome subunit 3 antibody
    • JAB1 containing signalosome subunit 3 antibody
    • JAB1-containing signalosome subunit 3 antibody
    • SGN 3 antibody
    • SGN3 antibody
    • Signalosome subunit 3 antibody
    see all

Anti-COPS3 antibody images

  • Lane 1 : Anti-COPS3 antibody (ab113365) at 1 µg/ml
    Lane 2 : Anti-COPS3 antibody (ab113365) at 1 mg/ml
    Lane 3 : anti-DYKDDDDK tag at 1/5000 dilution

    Lane 1 : HEK293 lysate (in RIPA buffer) overexpressing Human COPS3 with DYKDDDDK tag
    Lane 2 : Mock transfected HEK293 lysate
    Lane 3 : HEK293 lysate (in RIPA buffer) overexpressing Human COPS3 with DYKDDDDK tag

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Predicted band size : 48 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

References for Anti-COPS3 antibody (ab113365)

ab113365 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"