The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/20 - 1/200.
1/1000 - 1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
1/200 - 1/2000.
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
Widely expressed. Expressed at high level in heart and skeletal muscle.
Belongs to the CSN3 family. Contains 1 PCI domain.
All lanes : Anti-COPS3 antibody (ab229807) at 1/1000 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) whole cell lysate Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate Lane 4 : HT-29 (human colorectal adenocarcinoma cell line) whole cell lysate Lane 5 : Mouse heart lysate Lane 6 : Mouse brain lysate Lane 7 : Mouse ovarian lysate
Secondary All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution