• Product nameAnti-COPS3 antibody [EPR3127]
    See all COPS3 primary antibodies
  • Description
    Rabbit monoclonal [EPR3127] to COPS3
  • Tested applicationsSuitable for: WB, IP, Flow Cyt, ICC/IFmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) from the C-terminus

  • Positive control
    • HT29 cell lysate HeLa cells SKBR-3 cell lysate 293T cell lysate
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Alternative versions available:

    Anti-COPS3 antibody (Alexa Fluor® 488) [EPR3127] (ab207637)

    Anti-COPS3 antibody (Alexa Fluor® 647) [EPR3127] (ab207519)



Our Abpromise guarantee covers the use of ab79698 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/20000. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).
IP 1/40.
Flow Cyt 1/20 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/250 - 1/500.
  • Application notesIs unsuitable for IHC-P.
  • Target

    • FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
    • Tissue specificityWidely expressed. Expressed at high level in heart and skeletal muscle.
    • Sequence similaritiesBelongs to the CSN3 family.
      Contains 1 PCI domain.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Constitutive photomorphogenic homolog subunit 3 antibody
      • COP 9 (constitutive photomorphogenic) subunit 3 antibody
      • COP 9 complex homolog subunit 3 antibody
      • COP 9 complex S3 antibody
      • COP 9 complex subunit 3 antibody
      • COP 9 constitutive photomorphogenic homolog subunit 3 antibody
      • COP 9 homolog antibody
      • COP 9 signalosome complex subunit 3 antibody
      • COP 9 signalosome subunit 3 antibody
      • COP 9 subunit 3 antibody
      • COP9 (constitutive photomorphogenic) subunit 3 antibody
      • COP9 complex homolog subunit 3 antibody
      • COP9 complex S3 antibody
      • COP9 complex subunit 3 antibody
      • COP9 constitutive photomorphogenic homolog subunit 3 antibody
      • COP9 homolog antibody
      • COP9 signalosome complex subunit 3 antibody
      • COP9 signalosome subunit 3 antibody
      • COP9 subunit 3 antibody
      • COPS 3 antibody
      • cops3 antibody
      • CSN 3 antibody
      • CSN3 antibody
      • CSN3_HUMAN antibody
      • JAB 1 containing signalosome subunit 3 antibody
      • JAB1 containing signalosome subunit 3 antibody
      • JAB1-containing signalosome subunit 3 antibody
      • SGN 3 antibody
      • SGN3 antibody
      • Signalosome subunit 3 antibody
      see all

    Anti-COPS3 antibody [EPR3127] images

    • Immunofluorescence staining of HeLa cells with purified ab79698 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.

    • All lanes : Anti-COPS3 antibody [EPR3127] (ab79698) at 1/20000 dilution

      Lane 1 : HT-29 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : SKBR-3 cell lysate
      Lane 4 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 48 kDa
      Observed band size : 48 kDa
    • Overlay histogram showing HeLa cells stained with ab79698 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab79698, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    References for Anti-COPS3 antibody [EPR3127] (ab79698)

    This product has been referenced in:
    • Füzesi-Levi MG  et al. Dynamic regulation of the COP9 signalosome in response to DNA damage. Mol Cell Biol 34:1066-76 (2014). WB ; Human . Read more (PubMed: 24421388) »
    • Korczeniewska J & Barnes BJ The COP9 signalosome interacts with and regulates interferon regulatory factor 5 protein stability. Mol Cell Biol 33:1124-38 (2013). Read more (PubMed: 23275442) »

    See all 2 Publications for this product

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    I can inform you that the immunogens for the requested antibodies are as follows:

    Anti-COPS3 antibody [EPR3127] (ab79698)

    Synthetic peptide taken from within the region 380 to the C-terminusof ...

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