Overview

  • Product nameAnti-COPS6 antibody
    See all COPS6 primary antibodies
  • Description
    Rabbit polyclonal to COPS6
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Cow, Human, Pig, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide derived from within residues 150 - 250 of Human COPS6.

    (Peptide available as ab92686.)

  • Positive control
    • This antibody gave a positive signal in NIH3T3 whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77299 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
WB
  • Application notesICC/IF: Use at a concentration of 5 µg/ml.
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Has some glucocorticoid receptor-responsive activity.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the peptidase M67A family. CSN6 subfamily.
      Contains 1 MPN (JAB/Mov34) domain.
    • Cellular localizationNucleus. Cytoplasm. The interaction with HIV-1 Vpr protein possibly leads its translocation to a perinuclear region.
    • Information by UniProt
    • Database links
    • Alternative names
      • COP9 (constitutive photomorphogenic), subunit 6; COP9 complex S6 antibody
      • COP9 constitutive photomorphogenic homolog subunit 6 (Arabidopsis) antibody
      • COP9 signalosome complex subunit 6 antibody
      • COP9 signalosome subunit 6 antibody
      • COP9 subunit 6 (MOV34 homolog, 34 kD) antibody
      • cops6 antibody
      • CSN6 antibody
      • CSN6_HUMAN antibody
      • H_NH0506M12.12 antibody
      • hVIP antibody
      • JAB1 containing signalosome subunit 6 antibody
      • JAB1-containing signalosome subunit 6 antibody
      • MOV34 homolog antibody
      • MOV34 homolog, 34 kD antibody
      • MOV34-34KD antibody
      • OTTMUSP00000025868 antibody
      • Sgn3 antibody
      • SGN6 antibody
      • Signalosome subunit 6 antibody
      • VIP/MOV34 antibody
      • Vpr interacting protein antibody
      • Vpr-interacting protein antibody
      see all

    Anti-COPS6 antibody images

    • Anti-COPS6 antibody (ab77299) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 36 kDa
      Observed band size : 36 kDa


      Exposure time : 20 minutes
    • ICC/IF image of ab77299 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77299, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293 and HepG2 cells at 5µg/ml, and in 4% PFA fixed (10 min) HeLa, Hek293, HepG2 and MCF7 cells at 5µg/ml.

    References for Anti-COPS6 antibody (ab77299)

    ab77299 has not yet been referenced specifically in any publications.

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