Overview

  • Product nameAnti-COPS8 antibodySee all COPS8 primary antibodies ...
  • Description
    Rabbit polyclonal to COPS8
  • Tested applicationsWB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human COPS8.

    (Peptide available as ab93795.)

  • Positive control
    • This antibody gave a positive signal in following human lysates: Brain tissue, HepG2 whole cell, SHSY5Y whole cell, SAOS2 whole cell.

Properties

Applications

Our Abpromise guarantee covers the use of ab77300 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).Can be blocked with COPS8 peptide (ab93795).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
  • Sequence similaritiesBelongs to the CSN8 family.
    Contains 1 PCI domain.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • COP9 homolog antibody
    • COP9 signalosome complex subunit 8 antibody
    • Cops8 antibody
    • CSN8 antibody
    • CSN8_HUMAN antibody
    • hCOP9 antibody
    • JAB1 containing signalosome subunit 8 antibody
    • JAB1-containing signalosome subunit 8 antibody
    • SGN8 antibody
    • Signalosome subunit 8 antibody
    see all

Anti-COPS8 antibody images

  • All lanes : Anti-COPS8 antibody (ab77300) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
    Lane 3 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell Lysate
    Lane 4 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 23 kDa
    Observed band size : 23 kDa
    Additional bands at : 120 kDa,49 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • ICC/IF image of ab77300 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab77300, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) Hek293, HepG2 and MCF7 cells at 1µg/ml.

References for Anti-COPS8 antibody (ab77300)

ab77300 has not yet been referenced specifically in any publications.

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