Overview

  • Product nameAnti-COPS8 antibody [EPR5139]
    See all COPS8 primary antibodies
  • Description
    Rabbit monoclonal [EPR5139] to COPS8
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus of COPS8 (Human)

  • Positive control
    • WB: Fetal liver, HepG2, SH SY5Y or HeLa cell lysates IHC-P: Human brain tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-COPS8 antibody (Alexa Fluor® 647) [EPR5139] (ab207369)
    Anti-COPS8 antibody (Alexa Fluor® 488) [EPR5139] (ab208389)

     

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

Applications

Our Abpromise guarantee covers the use of ab124779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 21 kDa (predicted molecular weight: 23 kDa).
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Antigen retrieval is recommended.
ICC 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionComponent of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively.
    • Sequence similaritiesBelongs to the CSN8 family.
      Contains 1 PCI domain.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • COP9 homolog antibody
      • COP9 signalosome complex subunit 8 antibody
      • COP9 signalosome subunit 8 antibody
      • Cops8 antibody
      • CSN8 antibody
      • CSN8_HUMAN antibody
      • hCOP9 antibody
      • JAB1 containing signalosome subunit 8 antibody
      • JAB1-containing signalosome subunit 8 antibody
      • SGN8 antibody
      • Signalosome subunit 8 antibody
      see all

    Anti-COPS8 antibody [EPR5139] images

    • ab124779, at 1/100, staining COPS8 in formalin fixed paraffin embedded Human brain tissue using immunohistochemistry.
    • All lanes : Anti-COPS8 antibody [EPR5139] (ab124779) at 1/1000 dilution

      Lane 1 : Fetal liver cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : SH SY5Y cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size : 23 kDa
      Observed band size : 21 kDa (why is the actual band size different from the predicted?)

    References for Anti-COPS8 antibody [EPR5139] (ab124779)

    ab124779 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab124779.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"