Overview

  • Product name
  • Description
    Rabbit polyclonal to COQ4
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: ERPRISTSTL DLGKLQSLPE GSLGREYLRF LDVNRVSPDT RAPTRFVDDE ELAYVIQRYR EVHDMLHTLL GMPTNILGEI VVKW corresponding to amino acids 101-183 of Human COQ4 (UniProt Q9Y3A0).

  • Positive control
    • Human pancreas tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab126295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration.

Target

  • Function
    Component of the coenzyme Q biosynthetic pathway. May play a role in organizing a multi-subunit COQ enzyme complex required for coenzyme Q biosynthesis. Required for steady-state levels of other COQ polypeptides.
  • Tissue specificity
    Expressed ubiquitously, but at high levels in liver, lung and pancreas.
  • Pathway
    Cofactor biosynthesis; ubiquinone biosynthesis.
  • Sequence similarities
    Belongs to the COQ4 family.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • CGI 92 antibody
    • Coenzyme Q biosynthesis protein 4 homolog antibody
    • coq4 antibody
    • COQ4_HUMAN antibody
    • mitochondrial antibody
    • Ubiquinone biosynthesis protein COQ4 homolog antibody
    see all

Images

  • Lane 1: Marker [kDa] 250; 130; 95; 72; 55; 36; 28; 17; 10
    Lane 2: Negative control (vector only transfected HEK293T lysate)
    Lane 3: Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells.
  • ab126295, at a 1/1000 dilution, staining COQ4 in paraffin embedded Human pancreas tissue by Immunohistochemistry.

References

ab126295 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Horse Tissue lysate - whole (Skeletal Muscle)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
40 µg
Specification
Skeletal Muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

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Verified customer

Submitted May 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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