Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab78662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
IHC-P Use a concentration of 1 µg/ml.

Target

  • FunctionBinds and transactivates the sequence 5'-TAATC[CA]-3' which is found upstream of several photoreceptor-specific genes, including the opsin genes. Acts synergistically with other transcription factors, e.g. NRL and RX, to regulate photoreceptor cell-specific gene transcription. Essential for the maintenance of mammalian photoreceptors.
  • Tissue specificityRetina.
  • Involvement in diseaseDefects in CRX are the cause of Leber congenital amaurosis type 7 (LCA7) [MIM:613829]. LCA designates a clinically and genetically heterogeneous group of childhood retinal degenerations, generally inherited in an autosomal recessive manner. Affected infants have little or no retinal photoreceptor function as tested by electroretinography. LCA represents the most common genetic cause of congenital visual impairment in infants and children.
    Defects in CRX are the cause of cone-rod dystrophy type 2 (CORD2) [MIM:120970]; also known as cone-rod retinal dystrophy 2 (CRD2). CORDs are inherited retinal dystrophies belonging to the group of pigmentary retinopathies. CORDs are characterized by retinal pigment deposits visible on fundus examination, predominantly in the macular region, and initial loss of cone photoreceptors followed by rod degeneration. This leads to decreased visual acuity and sensitivity in the central visual field, followed by loss of peripheral vision. Severe loss of vision occurs earlier than in retinitis pigmentosa.
    Defects in CRX are a cause of retinitis pigmentosa (RP) [MIM:268000]. RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
  • Sequence similaritiesBelongs to the paired homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cone rod homeobox antibody
    • Cone rod homeobox protein antibody
    • Cone rod homeobox-containing gene antibody
    • cone-rod homeobox antibody
    • Cone-rod homeobox protein antibody
    • CORD 2 antibody
    • CRD antibody
    • CRX antibody
    • CRX_HUMAN antibody
    • LCA 7 antibody
    • LCA7 antibody
    • Orthodenticle homeobox 3 antibody
    • OTX 3 antibody
    • OTX3 antibody
    see all

Anti-CORD2 antibody images

  • Anti-CORD2 antibody (ab78662) at 1 µg/ml + Rat Retina Tissue Lysate at 10 µg

    Secondary
    Rabbit monoclonal [MC29] to PKC mu (phospho S916) (ab78080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Additional bands at : 18 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 3 minutes
  • IHC image of CORD2 staining in Mouse Eye FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab78662, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References for Anti-CORD2 antibody (ab78662)

ab78662 has not yet been referenced specifically in any publications.

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