Overview

  • Product nameAnti-CoREST antibody
    See all CoREST primary antibodies
  • Description
    Rabbit polyclonal to CoREST
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 432-481 (EVLQEWEAEH GKEETNGPSN QKPVKSPDNS IKMPEEEDEA PVLDVRYASA) of Human CoREST (NP_055971).

  • Positive control
    • HT1080 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab90962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 53 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionEssential component of the BHC complex, a corepressor complex that represses transcription of neuron-specific genes in non-neuronal cells. The BHC complex is recruited at RE1/NRSE sites by REST and acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. In the BHC complex, it serves as a molecular beacon for the recruitment of molecular machinery, including MeCP2 and SUV39H1, that imposes silencing across a chromosomal interval. Plays a central role in demethylation of Lys-4 of histone H3 by promoting demethylase activity of KDM1A on core histones and nucleosomal substrates. It also protects KDM1A from the proteasome.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the CoREST family.
    Contains 1 ELM2 domain.
    Contains 2 SANT domains.
  • DomainThe SANT domains may bridge the nucleosomal substrates and the demethylase KDM1A.
  • Post-translational
    modifications
    Phosphorylated by HSV-1 protein kinases in case of infection.
  • Cellular localizationNucleus. Upon infection by HSV-1, it is partially translocated into the cytoplasm in an HSV-1-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • COREST antibody
    • KIAA0071 antibody
    • Nr2b2 antibody
    • Nuclear receptor coregulator 1 antibody
    • Nuclear receptor subfamily 2 group B member 2 antibody
    • Protein CoREST antibody
    • RCOR antibody
    • Rcor1 antibody
    • RCOR1_HUMAN antibody
    • REST corepressor 1 antibody
    • Retinoic acid receptor RXR-beta antibody
    • Retinoid X receptor beta antibody
    • Rxrb antibody
    see all

Anti-CoREST antibody images

  • Anti-CoREST antibody (ab90962) at 1 µg/ml (in 5% skim milk / PBS buffer) + HT1080 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 53 kDa

References for Anti-CoREST antibody (ab90962)

ab90962 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"