Anti-CoREST3 / RCOR3 antibody (ab50197)

Overview

  • Product nameAnti-CoREST3 / RCOR3 antibody
    See all CoREST3 / RCOR3 primary antibodies
  • Description
    Rabbit polyclonal to CoREST3 / RCOR3
  • SpecificityThis antibody reacts with CoREST3/RCOR3
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Horse, Chicken, Cow, Cat, Dog
  • Immunogen

    A region within synthetic peptide: MRVGAEYQAR IPEFDPGATK YTDKDNGGML VWSPYHSIPD AKLDEYIAIA, corresponding to N terminal amino acids 1-50 of Human CoREST3/ RCOR3

  • Positive control
    • Jurkat cell lysate and human spleen tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab50197 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 47 kDa (predicted molecular weight: 56 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.
ELISA 1/62500.

Target

  • RelevanceThe CoREST3 gene encodes a functional corepressor required for regulation of neural specific gene expression. REST (RE1 silencing transcription factor 1) can mediate extraneuronal restriction by imposing either active repression via histone deacetylase recruitment or long term gene silencing using a distinct functional complex. Silencing of neuronal specific genes requires the recruitment of an associated corepressor, CoREST, that serves as a functional molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval, including transcriptional units that do not themselves contain REST response elements.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • 4921514E24Rik antibody
    • C730034D20Rik antibody
    • E130101E15Rik antibody
    • FLJ10876 antibody
    • FLJ16298 antibody
    • KIAA1343 antibody
    • MGC28186 antibody
    • OTTHUMP00000035053 antibody
    • RCOR3 antibody
    • REST corepressor 3 antibody
    • RP11-318L16.1 antibody
    see all

Anti-CoREST3 / RCOR3 antibody images

  • Anti-CoREST3 / RCOR3 antibody (ab50197) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 56 kDa
    Observed band size : 47 kDa (why is the actual band size different from the predicted?)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling CoREST3 / RCOR3 with ab50197 at 4-8µg/ml. Arrows indicate positive staining.

References for Anti-CoREST3 / RCOR3 antibody (ab50197)

ab50197 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (Whole Brain Homogenate)
Gel Running Conditions Reduced Denaturing (8% gel)
Loading amount 10 µg
Specification Whole Brain Homogenate
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Martin Engel

Verified customer

Submitted Nov 02 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"