Overview

  • Product nameAnti-CoREST3 / RCOR3 antibody
    See all CoREST3 / RCOR3 primary antibodies
  • Description
    Rabbit polyclonal to CoREST3 / RCOR3
  • Tested applicationsSuitable for: WB, IP, IHC-P, ICC, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
  • Immunogen

    Synthetic peptide corresponding to a region between residue 445 and 495 of human CoREST3/ RCOR3 (NP_060724.1), (and between residues 503-553 NP_001129695.1.).

  • Positive control
    • HeLa, 293T and NIH3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab76921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500.
ICC 1/50 - 1/250.

Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.

ICC/IF 1/50 - 1/250.

Target

  • RelevanceThe CoREST3 gene encodes a functional corepressor required for regulation of neural specific gene expression. REST (RE1 silencing transcription factor 1) can mediate extraneuronal restriction by imposing either active repression via histone deacetylase recruitment or long term gene silencing using a distinct functional complex. Silencing of neuronal specific genes requires the recruitment of an associated corepressor, CoREST, that serves as a functional molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval, including transcriptional units that do not themselves contain REST response elements.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • 4921514E24Rik antibody
    • C730034D20Rik antibody
    • E130101E15Rik antibody
    • FLJ10876 antibody
    • FLJ16298 antibody
    • KIAA1343 antibody
    • MGC28186 antibody
    • OTTHUMP00000035053 antibody
    • RCOR3 antibody
    • REST corepressor 3 antibody
    • RP11-318L16.1 antibody
    see all

Anti-CoREST3 / RCOR3 antibody images

  • Immunocytochemistry/Immunofluorescence analysis of NBF-fixed HeLa cells labelling CoREST3 / RCOR3 with ab76921 at 1/80 (2.5µg/ml). A DyLight® 488-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.
  • All lanes : Anti-CoREST3 / RCOR3 antibody (ab76921) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH3T3 whole cell lysate at 50 µg


    Predicted band size : 66 kDa
    Observed band size : 70 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 second
  • Detection of CoREST3 / RCOR3 by Western Blot of Immunprecipitate.
    ab76921 at 1µg/ml staining CoREST3 / RCOR3 in HeLa whole cell lysate immunoprecipitated using ab76921 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References for Anti-CoREST3 / RCOR3 antibody (ab76921)

This product has been referenced in:
  • Sáez JE  et al. Decreased Expression of CoREST1 and CoREST2 Together with LSD1 and HDAC1/2 during Neuronal Differentiation. PLoS One 10:e0131760 (2015). Read more (PubMed: 26111147) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"