Anti-CORO1B antibody (ab119714)
Key features and details
- Rabbit polyclonal to CORO1B
- Suitable for: WB, ICC/IF
- Reacts with: Rabbit, Human
- Isotype: IgG
Overview
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Product name
Anti-CORO1B antibody -
Description
Rabbit polyclonal to CORO1B -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Rabbit, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide, coupled to carrier protein, corresponding to amino acids at the C terminus of Human CORO1B.
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Positive control
- Human A431 and HeLa cell lysates; Mouse brain extracts and Rabbit spleen fibroblasts.
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General notes
Do not aliquot.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab119714 was affinity purified using CORO1B (C-terminus) peptide (without carrier). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 54 kDa.
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ICC/IF |
1/100.
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Notes |
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WB
1/1000. Predicted molecular weight: 54 kDa. |
ICC/IF
1/100. |
Target
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Function
Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction. -
Sequence similarities
Belongs to the WD repeat coronin family.
Contains 5 WD repeats. -
Post-translational
modificationsPhosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location. -
Cellular localization
Cytoplasm > cytoskeleton. Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers. - Information by UniProt
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Database links
- Entrez Gene: 57175 Human
- Entrez Gene: 100009415 Rabbit
- Entrez Gene: 29474 Rat
- Omim: 609849 Human
- SwissProt: Q9BR76 Human
- SwissProt: O89046 Rat
- Unigene: 6191 Human
- Unigene: 2819 Rat
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Alternative names
- COR1B_HUMAN antibody
- CORO 1B antibody
- CORO1B antibody
see all
Images
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Lanes 1-2 : Anti-CORO1B antibody (ab119714) at 1/1000 dilution
Lanes 3-4 : Anti-CORO1B (phospho S2) antibody (ab119705) at 1/1000 dilution
Lanes 1 & 3 : Human A431 cells treated with
Calyculin A (100 nM) for 30 min
Lanes 2 & 4 : Human A431 cells treated with
Calyculin A (100 nM) for 30 min before
treatment with lambda phosphatase
Predicted band size: 54 kDa -
ab119714 at 1/100 dilution staining CORO1B in Rabbit spleen fibroblasts treated with Calyculin A in the absence (left) or presence (right) of blocking peptide. Detection used an appropriate secondary antibody conjugated to Cy3.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab119714 has been referenced in 1 publication.
- Striepen JF & Voeltz GK Coronin 1C restricts endosomal branched actin to organize ER contact and endosome fission. J Cell Biol 221:N/A (2022). PubMed: 35802042