- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-CORO1B antibody
See all CORO1B primary antibodies -
DescriptionRabbit polyclonal to CORO1B
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Mouse, Rabbit, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide, coupled to carrier protein, corresponding to amino acids at the C terminus of Human CORO1B.
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Positive control
- Human A431 and HeLa cell lysates; Mouse brain extracts and Rabbit spleen fibroblasts.
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General notesDo not aliquot.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferPreservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab119714 was affinity purified using CORO1B (C-terminus) peptide (without carrier).
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab119714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 54 kDa. | |
| ICC/IF | 1/100. |
Target
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FunctionRegulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction.
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Sequence similaritiesBelongs to the WD repeat coronin family.
Contains 5 WD repeats. -
Post-translational
modificationsPhosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location. -
Cellular localizationCytoplasm > cytoskeleton. Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers.
- Information by UniProt
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Database links
- Entrez Gene: 57175 Human
- Entrez Gene: 23789 Mouse
- Entrez Gene: 100009415 Rabbit
- Entrez Gene: 29474 Rat
- Omim: 609849 Human
- SwissProt: Q9BR76 Human
- SwissProt: Q9WUM3 Mouse
- SwissProt: O89046 Rat
see all -
Alternative names
- COR1B_HUMAN antibody
- CORO 1B antibody
- CORO1B antibody
see all
Images
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Western blot - Anti-CORO1B antibody (ab119714)Lanes 1-2 : Anti-CORO1B antibody (ab119714) at 1/1000 dilution
Lanes 3-4 : Anti-CORO1B (phospho S2) antibody (ab119705) at 1/1000 dilution
Lanes 1 & 3 : Human A431 cells treated with
Calyculin A (100 nM) for 30 min
Lanes 2 & 4 : Human A431 cells treated with
Calyculin A (100 nM) for 30 min before
treatment with lambda phosphatase
Predicted band size: 54 kDa -
Immunocytochemistry - Anti-CORO1B antibody (ab119714)ab119714 at 1/100 dilution staining CORO1B in Rabbit spleen fibroblasts treated with Calyculin A in the absence (left) or presence (right) of blocking peptide. Detection used an appropriate secondary antibody conjugated to Cy3.
References
ab119714 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"