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Overview

  • Product name
    Anti-CORO1B antibody
    See all CORO1B primary antibodies
  • Description
    Rabbit polyclonal to CORO1B
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rabbit, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide, coupled to carrier protein, corresponding to amino acids at the C terminus of Human CORO1B.

  • Positive control
    • Human A431 and HeLa cell lysates; Mouse brain extracts and Rabbit spleen fibroblasts.
  • General notes
    Do not aliquot.

Properties

Applications

Our Abpromise guarantee covers the use of ab119714 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 54 kDa.
ICC/IF 1/100.

Target

  • Function
    Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction.
  • Sequence similarities
    Belongs to the WD repeat coronin family.
    Contains 5 WD repeats.
  • Post-translational
    modifications
    Phosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location.
  • Cellular localization
    Cytoplasm > cytoskeleton. Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • COR1B_HUMAN antibody
    • CORO 1B antibody
    • CORO1B antibody
    • Coronin 1B antibody
    • Coronin 2 antibody
    • Coronin actin binding protein 1B antibody
    • Coronin-1B antibody
    • Coronin-2 antibody
    • Coronin1B antibody
    • Coronin2 antibody
    • DKFZP762I166 antibody
    • OTTHUMP00000236823 antibody
    see all

Anti-CORO1B antibody images

  • Western blot - Anti-CORO1B antibody (ab119714)
    Western blot - Anti-CORO1B antibody (ab119714)
    Lanes 1 - 2 : Anti-CORO1B antibody (ab119714) at 1/1000 dilution
    Lanes 3 - 4 : Anti-CORO1B (phospho S2) antibody (ab119705) at 1/1000 dilution

    Lane 1 : Human A431 cells treated with Calyculin A (100 nM) for 30 min
    Lane 2 : Human A431 cells treated with Calyculin A (100 nM) for 30 min before treatment with lambda phosphatase
    Lane 3 : Human A431 cells treated with Calyculin A (100 nM) for 30 min
    Lane 4 : Human A431 cells treated with Calyculin A (100 nM) for 30 min before treatment with lambda phosphatase


    Predicted band size : 54 kDa
  • Immunocytochemistry - Anti-CORO1B antibody (ab119714)
    Immunocytochemistry - Anti-CORO1B antibody (ab119714)
    ab119714 at 1/100 dilution staining CORO1B in Rabbit spleen fibroblasts treated with Calyculin A in the absence (left) or presence (right) of blocking peptide. Detection used an appropriate secondary antibody conjugated to Cy3.

References for Anti-CORO1B antibody (ab119714)

ab119714 has not yet been referenced specifically in any publications.

Publishing research using ab119714? Please let us know so that we can cite the reference in this datasheet.

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