- Datasheet
- Specific References
- Protocols
Overview
- Product nameAnti-CORO1B antibody
See all CORO1B primary antibodies - DescriptionRabbit polyclonal to CORO1B
- Tested applicationsSuitable for: WB, ICC/IFmore details
- Species reactivityReacts with: Mouse, Rabbit, Human
Predicted to work with: Rat
- Immunogen
Synthetic peptide, coupled to carrier protein, corresponding to amino acids at the C terminus of Human CORO1B.
- Positive control
- Human A431 and HeLa cell lysates; Mouse brain extracts and Rabbit spleen fibroblasts.
- General notesDo not aliquot.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
- Storage bufferPreservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab119714 was affinity purified using CORO1B (C-terminus) peptide (without carrier).
- ClonalityPolyclonal
- IsotypeIgG
- Research areas
Associated products
- Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab119714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 54 kDa. | |
| ICC/IF | 1/100. |
Target
- FunctionRegulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction.
- Sequence similaritiesBelongs to the WD repeat coronin family.
Contains 5 WD repeats. - Post-translational
modificationsPhosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location. - Cellular localizationCytoplasm > cytoskeleton. Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers.
- Information by UniProt
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Database links
- Entrez Gene: 57175 Human
- Entrez Gene: 23789 Mouse
- Entrez Gene: 100009415 Rabbit
- Entrez Gene: 29474 Rat
- Omim: 609849 Human
- SwissProt: Q9BR76 Human
- SwissProt: Q9WUM3 Mouse
- SwissProt: O89046 Rat
see all -
Alternative names
- COR1B_HUMAN antibody
- CORO 1B antibody
- CORO1B antibody
see all
Anti-CORO1B antibody images
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Western blot - Anti-CORO1B antibody (ab119714)Lanes 1 - 2 : Anti-CORO1B antibody (ab119714) at 1/1000 dilution
Lanes 3 - 4 : Anti-CORO1B (phospho S2) antibody (ab119705) at 1/1000 dilution
Lane 1 : Human A431 cells treated with Calyculin A (100 nM) for 30 min
Lane 2 : Human A431 cells treated with Calyculin A (100 nM) for 30 min before treatment with lambda phosphatase
Lane 3 : Human A431 cells treated with Calyculin A (100 nM) for 30 min
Lane 4 : Human A431 cells treated with Calyculin A (100 nM) for 30 min before treatment with lambda phosphatase
Predicted band size : 54 kDa -
Immunocytochemistry - Anti-CORO1B antibody (ab119714)ab119714 at 1/100 dilution staining CORO1B in Rabbit spleen fibroblasts treated with Calyculin A in the absence (left) or presence (right) of blocking peptide. Detection used an appropriate secondary antibody conjugated to Cy3.
References for Anti-CORO1B antibody (ab119714)
ab119714 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"