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Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Purity Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
Use at 2-5 µg/mg of lysate.
1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Function Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction.
Sequence similarities Belongs to the WD repeat coronin family. Contains 5 WD repeats.
Post-translational modifications Phosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location.
Cellular localization Cytoplasm > cytoskeleton. Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers.
Information by UniProt
CORO 1B antibody
Anti-CORO1B antibody images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CORO1B antibody (ab99406)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling CORO1B with ab99406 at 1/1000 (0.2µg/ml). Detection: DAB.
Western blot - CORO1B antibody (ab99406)
All lanes : Anti-CORO1B antibody (ab99406) at 0.04 µg/ml Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Developed using the ECL technique Predicted band size : 54 kDa Exposure time : 10 seconds
Immunoprecipitation - CORO1B antibody (ab99406)
ab99406 at 1µg/ml detecting CORO1B in HeLa whole cell lysate by WB following IP.
Lane 1: ab99406 at 3µg/mg of lysate
Lane 2: IP with an antibody which recognizes an downstream epitope of CORO1B
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 3 seconds.
References for Anti-CORO1B antibody (ab99406)
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"