Overview

  • Product name
  • Description
    Rabbit polyclonal to CORO1B
  • Tested applications
    Suitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Corresponds to a region between amino acids 450-489 of Human CORO1B (NP_065174.1).

  • Positive control
    • HeLa, 293T and NIH 3T3 whole cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab99407 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 54 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Regulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction.
  • Sequence similarities
    Belongs to the WD repeat coronin family.
    Contains 5 WD repeats.
  • Post-translational
    modifications
    Phosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location.
  • Cellular localization
    Cytoplasm > cytoskeleton. Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • COR1B_HUMAN antibody
    • CORO 1B antibody
    • CORO1B antibody
    • Coronin 1B antibody
    • Coronin 2 antibody
    • Coronin actin binding protein 1B antibody
    • Coronin-1B antibody
    • Coronin-2 antibody
    • Coronin1B antibody
    • Coronin2 antibody
    • DKFZP762I166 antibody
    • OTTHUMP00000236823 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of humancolon carcinoma tissue labelling CORO1B with ab99407 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-CORO1B antibody (ab99407) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg
    Lane 5 : NIH 3T3 whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 54 kDa


    Exposure time : 3 seconds
  • ab99407 at 1µg/ml detecting CORO1B in HeLa whole cell lysate by WB following IP.

    Lane 1: IP with an antibody which recognizes an upstream epitope of CORO1B
    Lane 2: ab99407 at 3µg/mg of lysate
    Lane 3: control IgG.

    In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.

    Detection: Chemiluminescence with an exposure time of 1 second.

References

ab99407 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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