FunctionRegulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction.
Sequence similaritiesBelongs to the WD repeat coronin family. Contains 5 WD repeats.
Post-translational modificationsPhosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location.
Cellular localizationCytoplasm > cytoskeleton. Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of humancolon carcinoma tissue labelling CORO1B with ab99407 at 1/1000 (0.2µg/ml). Detection: DAB.
Western blot - CORO1B antibody (ab99407)
All lanes : Anti-CORO1B antibody (ab99407) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg Lane 2 : HeLa whole cell lysate at 15 µg Lane 3 : HeLa whole cell lysate at 5 µg Lane 4 : 293T whole cell lysate at 50 µg Lane 5 : NIH 3T3 whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size : 54 kDa
Exposure time : 3 seconds
Immunoprecipitation - CORO1B antibody (ab99407)
ab99407 at 1µg/ml detecting CORO1B in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of CORO1B
Lane 2: ab99407 at 3µg/mg of lysate
Lane 3: control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 1 second.
References for Anti-CORO1B antibody (ab99407)
has not yet been referenced specifically in any publications.
Publishing research using ab99407? Please let us know so that we can cite the reference in this datasheet.
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