Anti-CORO1B (phospho S2) antibody (ab119705)

Overview

  • Product nameAnti-CORO1B (phospho S2) antibody
    See all CORO1B primary antibodies
  • Description
    Rabbit polyclonal to CORO1B (phospho S2)
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Rabbit, Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phospho-peptide, coupled to carrier protein, corresponding to amino acids surrounding serine 2 of Human CORO1B.

  • Positive control
    • A431 and HelA cell lysateS, treated with Calyculin A; Rabbit spleen fibroblasts, treated with Calyculin A.
  • General notesDo not aliquot.

Properties

Applications

Our Abpromise guarantee covers the use of ab119705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 54 kDa.
ICC/IF 1/100.

Target

  • FunctionRegulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction.
  • Sequence similaritiesBelongs to the WD repeat coronin family.
    Contains 5 WD repeats.
  • Post-translational
    modifications
    Phosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location.
  • Cellular localizationCytoplasm > cytoskeleton. Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers.
  • Information by UniProt
  • Database links
  • Alternative names
    • COR1B_HUMAN antibody
    • CORO 1B antibody
    • CORO1B antibody
    • Coronin 1B antibody
    • Coronin 2 antibody
    • Coronin actin binding protein 1B antibody
    • Coronin-1B antibody
    • Coronin-2 antibody
    • Coronin1B antibody
    • Coronin2 antibody
    • DKFZP762I166 antibody
    • OTTHUMP00000236823 antibody
    see all

Anti-CORO1B (phospho S2) antibody images

  • Lanes 1 - 2 : Anti-CORO1B (C terminal) at 1/1000 dilution
    Lanes 3 - 4 : Anti-CORO1B (phospho S2) antibody (ab119705) at 1/1000 dilution

    Lane 1 : Human A431 cells treated with Calyculin A (100 nM) for 30 min
    Lane 2 : Human A431 cells treated with Calyculin A (100 nM) for 30 min before treatment with lambda phosphatase
    Lane 3 : Human A431 cells treated with Calyculin A (100 nM) for 30 min
    Lane 4 : Human A431 cells treated with Calyculin A (100 nM) for 30 min before treatment with lambda phosphatase


    Predicted band size : 54 kDa
  • ab119705 at 1/100 dilution staining CORO1B in Rabbit spleen fibroblasts treated with Calyculin A in the absence (left) or presence (right) of blocking peptide.
    Detection used an appropriate secondary antibody conjugated to Cy3.

References for Anti-CORO1B (phospho S2) antibody (ab119705)

ab119705 has not yet been referenced specifically in any publications.

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