The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
1/250 - 1/500.
FunctionMay play a role in the maintenance of the Golgi apparatus morphology and in the protein export from the Golgi.
Sequence similaritiesBelongs to the WD repeat coronin family. Contains 8 WD repeats.
Post-translational modificationsThe membrane-associated form is phosphorylated on tyrosine residues.
Cellular localizationCytoplasm > cytosol. Golgi apparatus membrane. Cytoplasmic vesicle. Predominantly cytosolic. Detected on vesicle-like cytoplasmic structures and on the cis-Golgi. Not associated with actin filaments.