Overview

  • Product nameAnti-Coronin 1a antibody
    See all Coronin 1a primary antibodies
  • Description
    Rabbit polyclonal to Coronin 1a
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide: RRRAAPEASG TPSSDAVSRL EEEMRKLQAT VQELQKRLDR LEETVQAK, corresponding to amino acids 414-461 of Human Coronin 1a

  • Positive control
    • Jurkat cell lysate.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab66374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/1562500.

WB Use a concentration of 1.25 µg/ml. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionMay be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.
  • Tissue specificityExpressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.
  • Sequence similaritiesBelongs to the WD repeat coronin family.
    Contains 7 WD repeats.
  • Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin binding protein antibody
    • CLABP antibody
    • Clipin A antibody
    • Clipin-A antibody
    • CLIPINA antibody
    • COR1A_HUMAN antibody
    • CORO1A antibody
    • Coronin 1 antibody
    • Coronin actin binding protein 1A antibody
    • Coronin like protein A antibody
    • Coronin like protein p57 antibody
    • Coronin-1A antibody
    • Coronin-like protein A antibody
    • Coronin-like protein p57 antibody
    • FLJ41407 antibody
    • HCORO1 antibody
    • MGC117380 antibody
    • OTTHUMP00000163017 antibody
    • p57 antibody
    • TACO antibody
    • Tryptophan aspartate containing coat protein antibody
    • Tryptophan aspartate-containing coat protein antibody
    see all

Anti-Coronin 1a antibody images

  • Anti-Coronin 1a antibody (ab66374) at 1.25 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 51 kDa
    Observed band size : 51 kDa
    Additional bands at : 33 kDa. We are unsure as to the identity of these extra bands.

References for Anti-Coronin 1a antibody (ab66374)

ab66374 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab66374.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"