Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab15719 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 20362548
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).Can be blocked with Human Coronin 3 peptide (ab23272).

Target

Anti-Coronin 3 antibody images



  • Predicted band size : 55 kDa
  • Anti-Coronin 3 antibody (ab15719) at 1/1000 dilution + Mouse 3T3 fibroblast whole cell lysate (25ug)

    Secondary
    HRP conjugated bovine anti-goat IgG

    Predicted band size : 55 kDa
    Observed band size : 55 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-Coronin 3 antibody (ab15719)

This product has been referenced in:
  • Kimura T  et al. Glucose-induced translocation of coronin 3 regulates the retrograde transport of the secretory membrane in the pancreatic beta-cells. Biochem Biophys Res Commun 395:318-23 (2010). ICC/IF ; Mouse . Read more (PubMed: 20362548) »
  • Kimura T  et al. The GDP-dependent Rab27a effector coronin 3 controls endocytosis of secretory membrane in insulin-secreting cell lines. J Cell Sci 121:3092-8 (2008). Read more (PubMed: 18768935) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (3T3 fibroblast)
Loading amount 25 µg
Specification 3T3 fibroblast
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
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Submitted Mar 21 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"