Anti-Coronin-1C antibody (ab15719)
Key features and details
- Goat polyclonal to Coronin-1C
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Coronin-1C antibody
See all Coronin-1C primary antibodies -
Description
Goat polyclonal to Coronin-1C -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Coronin-1C aa 450-550 (C terminal).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab15719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
A 1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). A 1 hour primary incubation is recommended for this product. |
Target
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Function
May be involved in cytokinesis, motility, and signal transduction. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the WD repeat coronin family.
Contains 4 WD repeats. - Information by UniProt
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Database links
- Entrez Gene: 23603 Human
- Omim: 605269 Human
- SwissProt: Q9ULV4 Human
- Unigene: 330384 Human
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Alternative names
- COR1C_HUMAN antibody
- CORO 1C antibody
- Coro1c antibody
see all
Images
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Ab15719 at 0.2µg/ml staining Coronin-1C in human lung lysate by Western blot (ECL)
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Anti-Coronin-1C antibody (ab15719) at 1/1000 dilution + Mouse 3T3 fibroblast whole cell lysate (25ug)
Secondary
HRP conjugated bovine anti-goat IgG
Predicted band size: 55 kDa
Observed band size: 55 kDa
This blot shows significant non-specific background staining. Increasing the length of the blocking step and including a blocking agent in the primary antibody solution may reduce this.
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab15719 has been referenced in 3 publications.
- Yang B et al. Proteomic screening and identification of microRNA-128 targets in glioma cells. Proteomics 15:2602-17 (2015). PubMed: 25781272
- Kimura T et al. Glucose-induced translocation of coronin 3 regulates the retrograde transport of the secretory membrane in the pancreatic beta-cells. Biochem Biophys Res Commun 395:318-23 (2010). ICC/IF ; Mouse . PubMed: 20362548
- Kimura T et al. The GDP-dependent Rab27a effector coronin 3 controls endocytosis of secretory membrane in insulin-secreting cell lines. J Cell Sci 121:3092-8 (2008). PubMed: 18768935