Overview

  • Product nameAnti-Coronin 3 antibody
    See all Coronin 3 primary antibodies
  • Description
    Mouse monoclonal to Coronin 3
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment: EEWFEGKNAD PILISLKHGY IPGKNRDLKV VKKNILDSKP TANKKCDLIS IPKKTTDTAS VQNEAKLDEI LKEIKSIKDT ICNQDERISK LEQQMAKIAA, corresponding to amino acids 375-474 of Human Coronin 3 (NP_055140) with proprietary tag.

  • Positive control
    • Hela Nuclear Extract. Recombinant tagged Human Coronin 3 fragment.

Properties

Applications

Our Abpromise guarantee covers the use of ab77203 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 53 kDa.
ELISA Use at an assay dependent dilution.

Target

Anti-Coronin 3 antibody images

  • Anti-Coronin 3 antibody (ab77203) at 1 µg/ml + Hela Nuclear extract at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 53 kDa
    Observed band size : 53 kDa
  • Anti-Coronin 3 antibody (ab77203) at 1 µg/ml + Recombinant tagged Human Coronin 3 fragment at 0.1 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

    Predicted band size : 53 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

References for Anti-Coronin 3 antibody (ab77203)

ab77203 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (gastric cancer cell lines and Hela cell line)
Loading amount 25 µg
Specification gastric cancer cell lines and Hela cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Gui Ren

Verified customer

Submitted Feb 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"