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Mixture of affinity purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60 src.
Our Abpromise guarantee covers the use of ab33333 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution. PubMed: 19704022|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 61 kDa. Use 20 µg per lane of 3T3 cell lysate as a positive control for mini gels.
Note: ab33333 recognizes 80kD and 85kD proteins in avian cells, but it recognizes an 80kD protein in rodent and human cells.
|IP||Use at 10 µg/mg of lysate.|
|ICC||Use a concentration of 10 µg/ml.|
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Blocking was with 5% milk for 1 hour at 20°C.