Overview

  • Product nameAnti-Cortactin antibody [0.T.21]
    See all Cortactin primary antibodies
  • Description
    Mouse monoclonal [0.T.21] to Cortactin
  • Tested applicationsSuitable for: ICC/IF, WB, IP, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Human
  • Immunogen

    Mixture of affinity purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60 src.

  • Positive control
    • WB: Mouse 3T3 cell lysate and chicken fibroblast src transformed cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab33333 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent dilution. PubMed: 19704022
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 61 kDa. Use 20 µg per lane of 3T3 cell lysate as a positive control for mini gels.
Note: ab33333 recognizes 80kD and 85kD proteins in avian cells, but it recognizes an 80kD protein in rodent and human cells.
IP Use at 10 µg/mg of lysate.
ICC Use a concentration of 10 µg/ml.

Target

Anti-Cortactin antibody [0.T.21] images

  • Anti-Cortactin antibody [0.T.21] (ab33333) at 1/1000 dilution + Bovine aortic endothelial whole cell lysates at 10 µg

    Secondary
    Goat anti-mouse polyclonal HRP conjugate at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 61 kDa
    Additional bands at : 80 kDa (possible post-translational modification).

    Exposure time : 5 seconds

    Image is courtesy of an anonymous AbReview

    Blocking was with 5% milk for 1 hour at 20°C.

    See Abreview

  • ab33333 staining Cortactin (red) in Human palladin-activated fibroblasts by Immunocytochemistry/ Immunofluorescence.

    Cells were fixed with paraformaldehyde and permeabilized with 0.2% Triton X-100. Samples were incubated with primary antibody (1/500 in diluent) before incubation with a Rhodamine Red IgG (1/100).

References for Anti-Cortactin antibody [0.T.21] (ab33333)

This product has been referenced in:
  • Weidmann MD  et al. Mena(INV) dysregulates cortactin phosphorylation to promote invadopodium maturation. Sci Rep 6:36142 (2016). Read more (PubMed: 27824079) »
  • Myers RB  et al. The matricellular protein CCN5 regulates podosome function via interaction with integrin avß 3. J Cell Commun Signal 8:135-46 (2014). Rat . Read more (PubMed: 24488697) »

See all 11 Publications for this product

Product Wall

Application Western blot
Sample African Green Monkey Cell lysate - whole cell (COS7 cell)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification COS7 cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Oct 16 2015

Application Western blot
Sample Dog Cell lysate - whole cell (Madin-Darby Canine Kidney (MDCK) epithelial cell)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification Madin-Darby Canine Kidney (MDCK) epithelial cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Sep 29 2015

Application Western blot
Sample Cow Cell lysate - whole cell (Bovine aortic endothelial cell)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification Bovine aortic endothelial cell
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Sep 25 2015

Application Western blot
Sample Human Cell lysate - whole cell (HEK293T cells)
Gel Running Conditions Reduced Denaturing
Loading amount 10 µg
Specification HEK293T cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Submitted Jun 19 2015

Application Immunocytochemistry/ Immunofluorescence
Blocking step BSA as blocking agent for 15 minute(s) · Concentration: 0.5% · Temperature: 20°C
Sample Mouse Cell (J774 macrophage)
Specification J774 macrophage
Permeabilization Yes - 0.05% saponin
Fixative Paraformaldehyde
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Submitted May 23 2014

Thank you for contacting us. I am sorry to hear that this antibody is not providing satisfactory results.

Would you be able to provide the lot number and order number?

It may be possible that the ˜100 kDa band corresponds to the pho...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"