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Mixture of affinity purified tyrosine phosphoproteins from chick embryo fibroblasts expressing activated pp60 src.
Our Abpromise guarantee covers the use of ab33333 in the following tested applications.
|ICC/IF||Use at an assay dependent dilution. PubMed: 19704022|
|WB||Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 61 kDa. Use 20 µg per lane of 3T3 cell lysate as a positive control for mini gels.
Note: ab33333 recognizes 80kD and 85kD proteins in avian cells, but it recognizes an 80kD protein in rodent and human cells.
|IP||Use at 10 µg/mg of lysate.|
|ICC||Use a concentration of 10 µg/ml.|
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Blocking was with 5% milk for 1 hour at 20°C.