Purification notesWhole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Predicted molecular weight: 65-75 kDa. This is determined using a rat brain cytosol extract, a whole extract of chicken embryo fibroblasts, or a whole cell extract of the human epidermoid carcinoma A431 cell line.
Use at an assay dependent dilution. PubMed: 20689769
Use at an assay dependent dilution. PubMed: 16971514
FunctionContributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
Spillane M et al. Nerve growth factor-induced formation of axonal filopodia and collateral branches involves the intra-axonal synthesis of regulators of the actin-nucleating Arp2/3 complex. J Neurosci32:17671-89 (2012).
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