Overview

  • Product name
  • Description
    Rabbit polyclonal to Cortactin
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Chicken, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to amino acids 361-380 of Human Cortactin.

  • Positive control
    • A431 cell line and NIH3T3 cell line.
  • General notes
    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.01M PBS, pH 7.4
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Whole antiserum is fractionated and then further purified by ion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab11066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 65-75 kDa.
ICC/IF Use at an assay dependent dilution.

Target

  • Function
    Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
  • Sequence similarities
    Contains 7 cortactin repeats.
    Contains 1 SH3 domain.
  • Domain
    The SH3 motif may mediate binding to the cytoskeleton.
  • Post-translational
    modifications
    Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amplaxin antibody
    • CTTN antibody
    • EMS 1 antibody
    • EMS1 antibody
    • FLJ34459 antibody
    • Mammary tumor and squamous cell carcinoma associated antibody
    • Oncogene EMS1 antibody
    • p80/85 src substrate antibody
    • Src substrate cortactin antibody
    • SRC8_HUMAN antibody
    see all

References

This product has been referenced in:
  • Xi W  et al. Molecular Insights into Division of Single Human Cancer Cells in On-Chip Transparent Microtubes. ACS Nano 10:5835-46 (2016). Read more (PubMed: 27267364) »
  • Høye AM  et al. The Phosphorylation and Distribution of Cortactin Downstream of Integrin a9ß1 Affects Cancer Cell Behaviour. Sci Rep 6:28529 (2016). Read more (PubMed: 27339664) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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