Overview

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris-saline. pH 7.3
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.01 - 0.03 µg/ml. Detects a band of approximately 80 kDa.Can be blocked with Human Cortactin peptide (ab22862).

Target

  • Function
    Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
  • Sequence similarities
    Contains 7 cortactin repeats.
    Contains 1 SH3 domain.
  • Domain
    The SH3 motif may mediate binding to the cytoskeleton.
  • Post-translational
    modifications
    Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
  • Cellular localization
    Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amplaxin antibody
    • CTTN antibody
    • EMS 1 antibody
    • EMS1 antibody
    • FLJ34459 antibody
    • Mammary tumor and squamous cell carcinoma associated antibody
    • Oncogene EMS1 antibody
    • p80/85 src substrate antibody
    • Src substrate cortactin antibody
    • SRC8_HUMAN antibody
    see all

Images

  • Anti-Cortactin antibody (ab1374) at 0.1 µg/ml + Lysate prepared from mouse brain tissue at 35 µg
    Developed using the ECL technique

    Primary incubated for 1 hour.

  • Anti-Cortactin antibody (ab1374) at 0.1 µg/ml + Human brain (cerebellum) tissue lysate at 35 µg

    Primary incubation was 1 hour. Detected by chemiluminescence

     

  • Anti-Cortactin antibody (ab1374) at 0.03 µg/ml + HEK293 cell lysate at 35 µg/ml

    Primary incubation was 1 hour. Detected by chemiluminescence

     

References

ab1374 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab1374.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up