• Product nameAnti-Cortactin antibody
    See all Cortactin primary antibodies
  • Description
    Rabbit polyclonal to Cortactin
  • Tested applicationsSuitable for: IHC-P, WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region within amino acids 450-500 of Human Cortactin (NP_005222.2).

  • Positive control
    • Whole cell lysate from HeLa cells and 293T cells


Associated products


Our Abpromise guarantee covers the use of ab93796 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/2000 - 1/10000. Predicted molecular weight: 62 kDa.
IP Use at 2-5 µg/mg of lysate.


  • FunctionContributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
  • Sequence similaritiesContains 7 cortactin repeats.
    Contains 1 SH3 domain.
  • DomainThe SH3 motif may mediate binding to the cytoskeleton.
  • Post-translational
    Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
  • Cellular localizationCytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amplaxin antibody
    • CTTN antibody
    • EMS 1 antibody
    • EMS1 antibody
    • FLJ34459 antibody
    • Mammary tumor and squamous cell carcinoma associated antibody
    • Oncogene EMS1 antibody
    • p80/85 src substrate antibody
    • Src substrate cortactin antibody
    • SRC8_HUMAN antibody
    see all

Anti-Cortactin antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling Cortactin with ab93796 at 1/1000 (0.2µg/ml). Detection: DAB.
  • All lanes : Anti-Cortactin antibody (ab93796) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    developed using the ECL technique

    Predicted band size : 62 kDa

    Exposure time : 10 seconds
  • Detection of Cortactin in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab93796 at 3 µg/mg lysate for IP (Lane 1) and at 0.4 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 1 seconds.

References for Anti-Cortactin antibody (ab93796)

ab93796 has not yet been referenced specifically in any publications.

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