ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Contributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
Mammary tumor and squamous cell carcinoma associated antibody
Oncogene EMS1 antibody
p80/85 src substrate antibody
Src substrate cortactin antibody
Western blot - Anti-Cortactin antibody [EP1922Y] (ab81208)
Lane 1: Wild-type HAP1 cell lysate (20 µg) Lane 2: Cortactin knockout HAP1 cell lysate (20 µg) Lane 3: NIH3T3 cell lysate (20 µg) Lane 4: A431 cell lysate (20 µg) Lanes 1 - 4: Merged signal (red and green). Green - ab81208 observed at 62 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab81208 was shown to specifically react with Cortactin in wild-type HAP1 cells along with additional cross reactive bands. No band was observed when Cortactin knockout samples were examined. Wild-type and Cortactin knockout samples were subjected to SDS-PAGE. ab81208 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
ab81208 staining Cortactin in wild-type HAP1 cells (top panel) and Cortactin knockout HAP1 cells (bottom panel). The cells were fixed with 4% formaldehyde (10min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab81208 at 1/1000 dilution and ab195889 at 1/250 dilution (shown in pseudo colour red) overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Western blot - Cortactin antibody [EP1922Y] (ab81208)
Anti-Cortactin antibody [EP1922Y] (ab81208) at 1/100000 dilution + HeLa cell lysate at 10 µg
Secondary HRP labelled Goat anti-Rabbit at 1/2000 dilution
Overlay histogram showing HeLa cells stained with ab81208 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab81208, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Chen Q et al. Growth-induced stress enhances epithelial-mesenchymal transition induced by IL-6 in clear cell renal cell carcinoma via the Akt/GSK-3ß/ß-catenin signaling pathway. Oncogenesis6:e375 (2017).
Read more (PubMed: 28846080) »
Sánchez A et al. Cyclic expression of the voltage-gated potassium channel KV10.1 promotes disassembly of the primary cilium. EMBO Rep17:708-23 (2016).
Read more (PubMed: 27113750) »