Anti-Cortactin (phospho Y421) antibody (ab47768)

Overview

  • Product nameAnti-Cortactin (phospho Y421) antibody
    See all Cortactin primary antibodies
  • Description
    Rabbit polyclonal to Cortactin (phospho Y421)
  • SpecificityThis antibody detects Cortactin only when phosphorylated at tyrosine 421.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized phosphopeptide derived from human Cortactin around the phosphorylation site of tyrosine 421 (P-I-YP-E-D).

  • Positive control
    • Hela cell extract.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab47768 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 85 kDa (predicted molecular weight: 62 kDa).
ELISA 1/20000.

Target

  • FunctionContributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
  • Sequence similaritiesContains 7 cortactin repeats.
    Contains 1 SH3 domain.
  • DomainThe SH3 motif may mediate binding to the cytoskeleton.
  • Post-translational
    modifications
    Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
  • Cellular localizationCytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
  • Information by UniProt
  • Database links
  • Alternative names
    • Amplaxin antibody
    • CTTN antibody
    • EMS 1 antibody
    • EMS1 antibody
    • FLJ34459 antibody
    • Mammary tumor and squamous cell carcinoma associated antibody
    • Oncogene EMS1 antibody
    • p80/85 src substrate antibody
    • Src substrate cortactin antibody
    • SRC8_HUMAN antibody
    see all

Anti-Cortactin (phospho Y421) antibody images

  • All lanes : Anti-Cortactin (phospho Y421) antibody (ab47768) at 1/500 dilution

    Lane 1 : Hela cell extract, treated with H2O2
    Lane 2 : Hela cell extract, untreated


    Predicted band size : 62 kDa

References for Anti-Cortactin (phospho Y421) antibody (ab47768)

This product has been referenced in:
  • Meiler E  et al. Cortactin tyrosine phosphorylation promotes its deacetylation and inhibits cell spreading. PLoS One 7:e33662 (2012). WB . Read more (PubMed: 22479425) »
  • García-Castillo J  et al. HER2 carboxyl-terminal fragments regulate cell migration and cortactin phosphorylation. J Biol Chem 284:25302-13 (2009). WB ; Human . Read more (PubMed: 19589785) »

See all 2 Publications for this product

Product Wall

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It may be possible that the ˜100 kDa band corresponds to the pho...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"