Overview

  • Product nameCorticosterone ELISA kit
  • Detection methodColorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 10.6%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 0.28 ng/ml
  • Range
    2 ng/ml - 22 ng/ml
  • Recovery

    = 101 %

  • Assay time
    3h 00m
  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Baboon
  • Product overview

    Abcam’s Corticosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Corticosterone levels in plasma, serum, urine, milk, saliva and cell culture supernatant.

    A Corticosterone specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Corticosterone is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Corticosterone captured in plate.

  • Tested applicationsSuitable for: Competitive ELISAmore details
  • PlatformMicroplate

Properties

  • RelevanceCorticosterone is the adrenal steroid, the major glucocorticoid. Glucocorticoid hormones are also known as corticosteroid hormones and are synthesized mainly in the adrenal cortex; however, more recently the enzymes involved in their synthesis have been found in a variety of cells and tissues, including the heart. The effects of these hormones are mediated via both cytoplasmic mineralocorticoid receptors (MRs) and glucocorticoid receptors (GRs), which act as ligand-inducible transcription factor. Corticosterone has profound effect on the structure and function of the hippocampus. Brain corticosterone action through the glucocorticoid receptor may involve memory storage. Emotional stress might cause increases in plasma corticosterone.

Applications

Our Abpromise guarantee covers the use of ab108821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Corticosterone ELISA kit images

  • Competitive ELISA with ab108821.
  • Corticosterone measured in biological fluids showing quantity (ng) per mL of tested sample

Protocols

References for Corticosterone ELISA kit (ab108821)

This product has been referenced in:
  • Medwid S  et al. Prenatal exposure to bisphenol A disrupts adrenal steroidogenesis in adult mouse offspring. Environ Toxicol Pharmacol 43:203-8 (2016). Mouse . Read more (PubMed: 27017381) »
  • Lee B  et al. Effects of systemic administration of ibuprofen on stress response in a rat model of post-traumatic stress disorder. Korean J Physiol Pharmacol 20:357-66 (2016). Read more (PubMed: 27382352) »

See all 13 Publications for this product

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J’ai effectué une extraction du corticostérone de fèces provenant de babouins Papio Anubis selon le protocole décrit dans l’article suivant « Monkeys in the Middle : Parasite Transmission through the Social Network of a Wild Primate » , Andrew J.J. Maclntosh,2012.
(J’ai cuis les fèces dans un four à 60° pendant 3h pour 1 g de fèces).
Ensuite j’ai utilisé le kit ab108821 de abcam pour doser la corticostérone.
J’ai testé différentes dilutions et il en sort qu’on peut utiliser l’échantillon non dilué.
J’ai obtenu des résultats qui correspondent au moyenne à une concentration de 4.52 ng/ml.
Par contre au niveau des contrôles j’ai obtenu des résultats que je ne comprends pas :
-Dans le premier contrôle je mets tout saut l’Ag et j’obtiens un très forte Abs, ce qui correspond à une très faible concentration – c’est normal.
-Dans le 2ème contrôle je mets tout sauf le 2ème AcI (l’Ag utilisé est celui correspondant au point 1 de la courbe standard [100ng/ml]) et là j’obtient une Abs proche d’une Abs correspondant à la concentration [100ng/ml]).
-Dans un 3ème contrôle je mets tout sauf l’AcII (l’Ag utilisé est celui correspondant au point 1 de la courbe standard [100ng/ml]) et là j’obtiens une Abs très faible correspondant à une concentration très forte.
-Dans un 4ème contrôle je mets uniquement du diluant et là j’obtiens une Abs très faible correspondant à une concentration très forte comme dans le contrôle précédent.

Corticosterone was extracted from feces of Papio Anubis baboons according to the protocol described in the following article : « Monkeys in the Middle : Parasite Transmission through the Social Network of a Wild Primate » , Andrew J.J. Maclntosh,2012.
(I cook feces in an oven at 60 ° for 3 h per 1 g of feces).
Then I used the kit ab108821 from Abcam to quantify the corticosterone.
I tested different dilutions and found out undiluted sample can be used.
I determined an average concentration of 4.52 ng/ml.
However in the controls I obtained results I do not understand:
- In the first test I put all jump Ag and I get a very strong Abs, which corresponds to a very low concentration - this is normal.
- In the second control I put everything except the second ACI (Ag used is the one corresponding to the point of the standard curve [100ng/ml]) and then I get a close Abs Abs corresponding to a concentration [100ng/ml]).
- In a third control I put everything except the ACII (Ag used is the one corresponding to the point of the standard curve [100ng/ml]) and then I get a very low Abs corresponding to a very high concentration.
- In a fourth-control I put only the diluent and then I get a very low Abs corresponding to a high concentration as in the previous test.
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Submitted Jun 18 2013

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