Overview

  • Product name
    Corticosterone ELISA kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 10.6%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 0.28 ng/ml
  • Range
    2 ng/ml - 22 ng/ml
  • Recovery

    = 101 %

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Baboon
  • Product overview

    Corticosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab108821) is designed for the quantitative measurement of Corticosterone levels in plasma, serum, urine, milk, saliva and cell culture supernatant.


    A Corticosterone specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Corticosterone is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Corticosterone captured in plate.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

  • Relevance
    Corticosterone is the adrenal steroid, the major glucocorticoid. Glucocorticoid hormones are also known as corticosteroid hormones and are synthesized mainly in the adrenal cortex; however, more recently the enzymes involved in their synthesis have been found in a variety of cells and tissues, including the heart. The effects of these hormones are mediated via both cytoplasmic mineralocorticoid receptors (MRs) and glucocorticoid receptors (GRs), which act as ligand-inducible transcription factor. Corticosterone has profound effect on the structure and function of the hippocampus. Brain corticosterone action through the glucocorticoid receptor may involve memory storage. Emotional stress might cause increases in plasma corticosterone.

Applications

Our Abpromise guarantee covers the use of ab108821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Corticosterone ELISA kit images

  • Competitive ELISA with ab108821.
  • Corticosterone measured in biological fluids showing quantity (ng) per mL of tested sample

Protocols

References for Corticosterone ELISA kit (ab108821)

This product has been referenced in:
  • Jafari Z  et al. Corticosterone response to gestational stress and postpartum memory function in mice. PLoS One 12:e0180306 (2017). ELISA ; Mouse . Read more (PubMed: 28692696) »
  • Jaggar M  et al. 5-HT2A receptor deficiency alters the metabolic and transcriptional, but not the behavioral, consequences of chronic unpredictable stress. Neurobiol Stress 7:89-102 (2017). Competitive ELISA ; Mouse . Read more (PubMed: 28626787) »

See all 17 Publications for this product

Product Wall

Unfortunately the components of this buffer are considered proprietary. And while this buffer has not been tested for use with protein assays, its likley that some of the components would cause erroneous results in a protein assay.



It is always good idea to run creatinine assay as a normalization factor for the urine sample since the urine concentration is varied significantly. Some of our customers do use a creatinine assay but others don't so it is really up to you. Read More

Yes. This kit is a competitive ELISA assay. Corticostone is competing with the biotinylated-corticosterone. The higher the concentration of corticosterone in sample, the lower OD.

Thank you for contacting us.

According to the protocol on our datasheet, this kit contains sealing tapes: Each kit contains 3 pre-cut, pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.

Rega...

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Thank you for contacting us. Since Corticosterone is stable molecule, we think it should work. But there is no guarantee since we have not tested this condition.

I hope this information is helpful to you. Please do not hesitate to contact us i...

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La source du kit m'a informé qu'ils n'avaient malheureusement jamais testé ce genre de kits sur des selles. Leur recommandation est d'essayer une série de dilutions au 1:2, 1:4, 1:8, etc.

J'espère que ces informatio...

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Je suis ravi que vous souhaitiez participer à notre offre et tester ab108821 sur selles de singe.
Le code de réduction correspondant est ****. Ce code de ré...

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Thank you for contacting us.

Unfortunately, this kit has not been tested for cross-reactivity with 11-DHC, so we do not have any information about whether or not it would cross-react. I am sorry I could not be more helpful, please let me kno...

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Thank you for contacting us.

In order to determine the sample concentration values use the standard curve equation. Substitute the values for the absorbances obtained in the equation and multiply the value by the dilution factor.

Ch...

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We are happy to help! :-)
I wish you success with your experiments and a good weekend.

1-10 of 19 Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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