Anti-Corticotropin Releasing Factor antibody [4H9] (ab35748)

Overview

  • Product name
    Anti-Corticotropin Releasing Factor antibody [4H9]
    See all Corticotropin Releasing Factor primary antibodies
  • Description
    Mouse monoclonal [4H9] to Corticotropin Releasing Factor
  • Specificity
    Reacts with CRF. Does not react with bovine serum albumin, insulin, ovalbumin or epidermal growth factor.
  • Tested applications
    Suitable for: ELISA, IHC-P, IHC-Fr, Inhibition Assaymore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat
  • Immunogen

    Full length protein (Human)

  • Epitope
    The unique lysine residue (Lys-36) is likely to be involved in the antigenic determinant of ab35748. Lysine modification by CA blocks binding completely.

Properties

Applications

Our Abpromise guarantee covers the use of ab35748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
IHC-Fr 1/100 - 1/7000.
Inhibition Assay Use at an assay dependent concentration. Human serum, containing CRF-binding protein, inhibits the binding of CRF to this antibody.

Target

  • Relevance
    CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).
  • Cellular localization
    Cytoplasmic and Secreted
  • Database links
  • Alternative names
    • Corticoliberin antibody
    • Corticotropin releasing factor antibody
    • Corticotropin releasing hormone antibody
    • CRF antibody
    • Crh antibody
    • CRH1 antibody
    see all

Anti-Corticotropin Releasing Factor antibody [4H9] images

  • ab35748 staining Corticotrophin Releasing Factor in Rat brain tissue by Immunohistochemistry (Frozen sections). The sections were fixed in methanol prior to blocking with PBS with 1.5% goat serum for 20 minutes at 22°C. The primary antibody was diluted 1/100 in the blocking buffer and incubated with the sample for 10 hours at 4°C. A Dylight® 594-conjugated polyclonal antibody was used as the secondary antibody, diluted 1/80.

    See Abreview

References for Anti-Corticotropin Releasing Factor antibody [4H9] (ab35748)

This product has been referenced in:
  • Kravchenko IV & Furalev VA Monoclonal antibodies directed against two different corticotropin-releasing factor determinants. Hybridoma 13:59-64 (1994). Read more (PubMed: 7515375) »

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Paraventricular nucleus brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Triton-X 100
Specification
Paraventricular nucleus brain
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Username

Dr. Fiona Shenton

Verified customer

Submitted Jun 26 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - Triton-x
Blocking step
Casein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 15 2012

Thank you for contacting us.

As we discussed on the phone, adding a BSA block and trying a shorter antigen retrieval time may help to reduce the background for ab7963, ab59023, and ab75168, while trying a 1:100 dilution for ab35748 may help t...

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Thank you for contacting us and your patience while I double checked with the laboratory.

The antibody will recognize an epitope around the Lysine 36 of the mature protein, as the lysine modification blocks the binding completely. The lysine ...

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Merci pour ces informations. Je devine qu'un démasquage de l'antigène a également été tenté à pH 6.0 comme recommandé par le laboratoire qui produit cet anticorps, voir mon email du 21 novembre 2011 ci-après. Pourriez-vous s'il vous plait me confir...

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Le laboratoire vient de m'informer que le tampon recommandé pour le démasquage de l'antigène est un tampon citrate pH 6,0. Placer les lames dans le tampon et chauffer au micro-ondes pendant 10 minutes. Laisser refroidir pendant 15 minutes et rincer ave...

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Thank you for contacting Abcam. Regarding ab35748, this monoclonal antibody was generated using a full length protein as the immunogen.  Unfortunately, epitope mapping has not been performed, although K36 is likely to be a part of the immunogen.  W...

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Thank you very much for contacting me again and for passing some useful information. Although, pig has not been tested for cross-reaction; ab35748 should recognize Corticotropin Releasing Factor in human samples. I can offer your troubl...

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Thank you for your enquiry regarding ab35748 and for taking the time to provide some useful details of the experiments. I am very sorry to hear that you are having problems with this antibody. Dr. Nitsan Kozlovsky has submitted an Abreview in March...

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Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (brain)
Specification
brain
Fixative
Methanol
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 22°C
Username

Dr. Nitsan Kozlovsky

Verified customer

Submitted Mar 02 2011

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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