Corticotropin Releasing Factor antibody (ab11133)

Product Name 

Corticotropin Releasing Factor antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to Corticotropin Releasing Factor

Immunogen 

Synthetic peptide: SQEPPISLDL TFHLLREVLE MTKADQLAQQ AHSNRKLLDI corresponding to amino acids 148-188 of Sheep CRF.
BLAST 'SQEPPISLDL TFHLLREVLE MTKADQLAQQ AHSNRKLLDI ' with or

Reacts with 

Hu, Rat, Shp

Predicted to react 
(species key)

Cow
(Due to sequence homology)

Specificity 

This antibody blocks specifically with the ovine CRF sequence that it was raised against. Cells and fibres are found in the paraventricular hypothalamic nucleus of rat brain in colchicine treated 4% FA perfused tissue.

Tested applications 
(see key)

IHC-Fr

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
IHC-Fr: 1/500 - 1/7500.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Secreted

Research areas 

Neuroscience >> Endocrine system >> hypothalamic pituitary adrenal axis
Signal Transduction >> Growth Factors/Hormones >> Hormones

Relevance 

CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).

Corticotropin-releasing hormone (CRH) is a 41-amino acid peptide derived from a 191-amino acid preprohormone. CRH is secreted by the paraventricular nucleus (PVN) of the hypothalamus in response to stress. Marked reduction in CRH has been observed in association with Alzheimer disease and autosomal recessive hypothalamic corticotropin dificiency has multiple and potentially fatal metabolic consequences including hypoglycemia and hepatitis. In addition to production in the hypothalamus, CRH is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. In the placenta CRH is a marker that determines the length of gestation and the timing of parturition and delivery. A rapid increase in circulating levels of CRH occurs at the onset of parturition, suggesting that, in addition to its metabolic functions, CRH may act as a trigger for parturition

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: 0.09% Sodium Azide
Constituents: PBS, Whole Serum.

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Notes 

This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.

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