Anti-Corticotropin Releasing Factor antibody (ab8901)

Overview

  • Product name
    Anti-Corticotropin Releasing Factor antibody
    See all Corticotropin Releasing Factor primary antibodies
  • Description
    Rabbit polyclonal to Corticotropin Releasing Factor
  • Tested applications
    Suitable for: ICC, RIA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 1-41 of Rat Corticotropin Releasing Factor.

Properties

Applications

Our Abpromise guarantee covers the use of ab8901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
AP Use at an assay dependent concentration.
ICC 1/1000.
RIA 1/600000.
WB 1/10000.
IHC-P 1/200 - 1/600.

Target

  • Relevance
    CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).
  • Cellular localization
    Cytoplasmic and Secreted
  • Database links
  • Alternative names
    • Corticoliberin antibody
    • Corticotropin releasing factor antibody
    • Corticotropin releasing hormone antibody
    • CRF antibody
    • Crh antibody
    • CRH1 antibody
    see all

Images

  • Immunohistochemical analysis of rat brain tissue, staining Corticotropin Releasing Factor with ab8901.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 10% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in diluent) for 24 hours at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody. The figure on the left is a secondary only control.

    See Abreview

  • Anti-Corticotropin Releasing Factor antibody (ab8901) at 1/2500 dilution + Mouse hypothalamus whole tissue lysate at 50 µg

    Secondary
    HRP-conjugated Donkey anti-rabbit IgG polyclonal - undiluted
    Developed using the ECL technique

    Performed under non-reducing conditions.

    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 17 kDa (possible non-specific binding),23 kDa (possible non-specific binding),35 kDa (possible non-specific binding).

    Exposure time : 1 minute

    This image is courtesy of an Abreview submitted by Jean-Christophe Helbling

    See Abreview

  • Ab8901 staining Corticotropin releasing factor in Human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/4000 in blocking buffer) for 16 hours at 21°C. A diluted Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Radioimmunoassay using ab8901
    - Day 1: Add Recombinant Protein A/G (10ug/ml) to a 96 wells plate; parafilm wrap; incubate overnight at 4°C.
    - Day 2: Aspirate and wash the plate to remove loosely bound Protein A/G; add antibody solution (dilution 1:50000); parafilm wrap; incubate 24h at 4°C.
    - Day 3: Aspirate and wash the plate to remove loosely bound antibody; add CRF standard (0, 0.03, 0.1, 0.3, 1, 3, 10, 30 ng/ml); parafilm wrap; incubate 24h at 4°C.
    - Day 4: DO NOT ASPIRATE; add 125I-CRF; incubate 24h at 4°C.
    - Day 5: Aspirate and wash the plate; break the plate and collect the wells in scintillation tubes; add 2ml of scintillation liquid; count in beta counter (open the 3H window to at least 30-40 keV), at least 2min/tube.

References

This product has been referenced in:
  • Feetham CH  et al. TRPV4 and K(Ca) ion channels functionally couple as osmosensors in the paraventricular nucleus. Br J Pharmacol 172:1753-68 (2015). Read more (PubMed: 25421636) »
  • Cuevas Guaman M  et al. Administration of antenatal glucocorticoids and postnatal surfactant ameliorates respiratory distress syndrome-associated neonatal lethality in Erk3(-/-) mouse pups. Pediatr Res 76:24-32 (2014). IHC ; Mouse . Read more (PubMed: 24732107) »

See all 7 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Placenta
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde
Username

Mr. Carl Hobbs

Verified customer

Submitted Mar 27 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Mouse Tissue lysate - whole (Hypothalamus)
Specification
Hypothalamus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Jean-Christophe Helbling

Verified customer

Submitted Sep 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - 0.01% Triton-X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Jan 04 2013

Thank you for sending us your customer's questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital...

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Thank you for contacting us.


The link to our conjugation kits and purifications kits is either http://www.abcam.com/EasyLink or
http://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=e...

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Thank you for your reply. I have published your Abreview on our online datasheet and awarded Abpoints to your user account. You can view and redeem your Abpoints by logging in to your account on our website.

Additionally, since you have submi...

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Thank you for contacting Abcam regarding ab8901.


The predicted molecular weight of this protein is 21kDa. In WB you should detect a band at approximately this size.


I hope this information is helpful. Please do not hesit...

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I am very pleased to hear you would like to use ab8901 in RIA and send us your data and an image of your results.

This code will give you 1 freeprimary antibody before the expiration date. To redeem this offer, please submit an Abreview with...

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Thank you for your reply.

I do apologize as I have been unable to obtain any more specific information regarding the dilution of this antibody to be used in RIA.

Due to this lack of specific details, I would like to offer you a te...

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Thank you for contacting Abcam regarding ab8901.


I have confirmed with the laboratory that RIA was not tested in house, but based on the following references:

1. Widmaier EP, Lim AT, Vale W. (1989) Secretion of corticotropin-...

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