Anti-Corticotropin Releasing Factor Receptor 2 antibody (ab104368)

Overview

  • Product nameAnti-Corticotropin Releasing Factor Receptor 2 antibody
    See all Corticotropin Releasing Factor Receptor 2 primary antibodies
  • Description
    Rabbit polyclonal to Corticotropin Releasing Factor Receptor 2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Horse, Hamster, Cow, Dog, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Rat Corticotropin Releasing Factor Receptor 2.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Rat Brain; Rat Forebrain; Rat Dorsal Root Ganglion; Mouse Dopaminergic Nucleus.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51,54 kDa (predicted molecular weight: 48 kDa).

Target

Anti-Corticotropin Releasing Factor Receptor 2 antibody images

  • All lanes : Anti-Corticotropin Releasing Factor Receptor 2 antibody (ab104368) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Rat Forebrain
    Lane 3 : Dorsal Root Ganglia (Rat) Tissue Lysate
    Lane 4 : Dopaminergic nucleus Mouse Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 48 kDa
    Observed band size : 51 + 54 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

References for Anti-Corticotropin Releasing Factor Receptor 2 antibody (ab104368)

ab104368 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Tissue lysate - whole (intestinal tissue)
Gel Running Conditions Reduced Denaturing (4-12% BIS TRIS)
Loading amount 28 µg
Specification intestinal tissue
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Julien Chevalier

Verified customer

Submitted May 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"