Anti-Corticotropin Releasing Factor Receptor 2 antibody (ab104368)

Overview

  • Product name
    Anti-Corticotropin Releasing Factor Receptor 2 antibody
    See all Corticotropin Releasing Factor Receptor 2 primary antibodies
  • Description
    Rabbit polyclonal to Corticotropin Releasing Factor Receptor 2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Horse, Hamster, Cow, Dog, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Rat Corticotropin Releasing Factor Receptor 2.

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Rat Brain; Rat Forebrain; Rat Dorsal Root Ganglion; Mouse Dopaminergic Nucleus.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 51,54 kDa (predicted molecular weight: 48 kDa).

Target

Images

  • All lanes : Anti-Corticotropin Releasing Factor Receptor 2 antibody (ab104368) at 1 µg/ml

    Lane 1 : Brain (Rat) Tissue Lysate
    Lane 2 : Rat Forebrain
    Lane 3 : Dorsal Root Ganglia (Rat) Tissue Lysate
    Lane 4 : Dopaminergic nucleus Mouse Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 48 kDa
    Observed band size: 51 + 54 kDa (why is the actual band size different from the predicted?)


    Exposure time: 30 seconds


    Corticotropin Releasing Factor Receptor 2 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. This antibody was raised against an immunogen that is predicted to recognize both alpha and beta isoforms of Corticotropin Releasing Factor Receptor 2. The predicted molecular weights of the alpha and beta isoforms are 48 kDa and 50 kDa respectively.

References

ab104368 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Human Tissue lysate - whole (intestinal tissue)
Gel Running Conditions
Reduced Denaturing (4-12% BIS TRIS)
Loading amount
28 µg
Specification
intestinal tissue
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Julien Chevalier

Verified customer

Submitted May 20 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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