The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 8 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
This is a receptor for corticotropin releasing factor. Shows high-affinity CRF binding. Also binds to urocortin I, II and III. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase.
Belongs to the G-protein coupled receptor 2 family.