Overview

  • Product nameAnti-Cortisol antibody [XM210]
    See all Cortisol primary antibodies
  • Description
    Mouse monoclonal [XM210] to Cortisol
  • SpecificityCortisol, Ka = 1.7 x 109 M-1. XM-210 has some cross-reactivity with cortisone (~0.6%)
  • Tested applicationsSuitable for: RIA, ELISAmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to Cortisol conjugated to Bovine Serum Albumin (BSA).

  • General notesConcentration varies from lot to lot and can be provided on request.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 10mM Tris. pH 7.5
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified by ion exchange chromatography and tested by elctrophoresis.
  • ClonalityMonoclonal
  • Clone numberXM210
  • MyelomaSp2/0
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA Use at an assay dependent concentration. PubMed: 23077165
ELISA Use at an assay dependent concentration.

Target

  • RelevanceCortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.
  • Cellular localizationSecreted

Anti-Cortisol antibody [XM210] images

  • Cross-reactivity, % 50% displacement of cortisol-3-125-Iodine.

References for Anti-Cortisol antibody [XM210] (ab1949)

This product has been referenced in:
  • Vindas MA  et al. Coping with unpredictability: dopaminergic and neurotrophic responses to omission of expected reward in Atlantic salmon (Salmo salar L.). PLoS One 9:e85543 (2014). RIA . Read more (PubMed: 24465595) »
  • Tsalafouta A  et al. Ontogenesis of the HPI axis and molecular regulation of the cortisol stress response during early development in Dicentrarchus labrax. Sci Rep 4:5525 (2014). ELISA . Read more (PubMed: 24984570) »

See all 4 Publications for this product

Product Wall

To our knowledge, ab1949 nor ab6066 have not yet been tested in detecting canine cortisol and testosterone. However, as cortisol and testosterone are steroids, antibodies raised against them should detect them regardless of the species that they are pr...

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Thank you for contacting us and your interest in our products.

We have two antibodies directed against cortisol which have been used in ELISA previously. These are the mouse monolonals ab1949 and ab116600. However these have not been used in a...

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Thank you for confirming that information.

The antibodies you have suggested for DHEA (ab54202, ab35062 and ab123987) should be fine for what the customer has requested.

The same is true of the anti-DHEA-S (ab31668). The anti-Cortis...

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Thank you for contacting us.

To be honest we do not have an antibody that we have tested with this kind of novel assay, and therefore do not know if it would work nor can we guarantee it.

Although ab1949 may work, I may recommend ab...

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Thanks for your inquiry. Yes ab1949 should work in mouse serum. However, there will likely be an issue with non-specific binding of your secondary antibody, which will be an anti-mouse IgG, with the IgG in the mouse serum. Of course, this is assu...

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Thank you for your enquiry. The immunogen is Cortisol-3(CMO)-BSA. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"