Overview

  • Product name
    Anti-Cortisol Binding Globulin antibody
    See all Cortisol Binding Globulin primary antibodies
  • Description
    Rabbit polyclonal to Cortisol Binding Globulin
  • Tested applications
    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to internal sequence amino acids 200-229 of Human Cortisol Binding Globulin (NP_001747.2).

  • Positive control
    • Mouse kidney tissue lysates IHC-P: human liver tissue sections. IF/ICC: HepG2 cell line.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab107368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 45 kDa.

Target

  • Function
    Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.
  • Tissue specificity
    Plasma; synthesized in liver. Has also been identified in a number of glycocorticoid responsive cells.
  • Involvement in disease
    Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency) [MIM:611489]. CBG deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue.
  • Sequence similarities
    Belongs to the serpin family.
  • Post-translational
    modifications
    N-glycosylated; binds 5 oligosaccharide chains.
    Glycosylation in position Asn-260 is needed for steroid binding.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • CBG antibody
    • CBG_HUMAN antibody
    • corticosteroid binding globulin antibody
    • Corticosteroid-binding globulin antibody
    • serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 6 antibody
    • Serpin A6 antibody
    • serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 6 antibody
    • Serpina6 antibody
    • Transcortin antibody
    see all

Images

  • ICC/IF image of ab107368 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab107368, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-Cortisol Binding Globulin antibody (ab107368) at 1/100 dilution + Mouse kidney tissue lysates at 35 µg

    Predicted band size : 45 kDa
  • IHC image of ab107368 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab107368, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:
  • Verhoog N  et al. Inhibition of corticosteroid-binding globulin gene expression by glucocorticoids involves C/EBPß. PLoS One 9:e110702 (2014). Read more (PubMed: 25335188) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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