Cortisol ELISA Kit (ab108665)
- Product nameCortisol ELISA KitSee all Cortisol kits ...
- Detection methodColorimetric
Intra-assay Sample n Mean SD CV% Overall 60 <=9%
- Tests1 x 96 well plate
- Sample typeSerum, Plasma
- Assay typeCompetitive
- Sensitivity= 2.44 ng/ml
- Range10 ng/ml - 500 ng/ml
- Assay durationMultiple steps standard assay
- Product overview
Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in serum and plasma.
A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where any Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.
- Tested applicationsCompetitive ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Washing Solution 1 x 50ml Anti-Cortisol (IgG) Coated Microplate (12 x 8 wells) 1 unit Cortisol Control 1 x 1ml Cortisol Standard 0 – 0 ng/mL 1 x 1ml Cortisol Standard 1 – 10 ng/mL 1 x 1ml Cortisol Standard 2 – 50 ng/mL 1 x 1ml Cortisol Standard 3 – 150 ng/mL 1 x 1ml Cortisol Standard 4 – 500 ng/mL 1 x 1ml Cortisol-HRP conjugate 1 x 21ml Stop Solution 1 x 15ml TMB Substrate Solution 1 x 15ml
- Research Areas
Our Abpromise guarantee covers the use of ab108665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for Cortisol ELISA Kit (ab108665)
ab108665 has not yet been referenced specifically in any publications.