Overview

  • Product nameCortisol ELISA KitSee all Cortisol kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Serum, Plasma
  • Assay typeCompetitive
  • Sensitivity
    = 2.44 ng/ml
  • Range
    10 ng/ml - 500 ng/ml
  • Recovery

    103.56 %

  • Assay durationMultiple steps standard assay
  • Product overview

    Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in serum and plasma.

     

    A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where any Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

  • Tested applicationsCompetitive ELISAmore details
  • PlatformMicroplate

Properties

    Applications

    Our Abpromise guarantee covers the use of ab108665 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Competitive ELISA Use at an assay dependent dilution.

    Cortisol ELISA Kit images

    • Cortisol measured in biological fluids showing quantity (ng) per mL of tested sample

    • Representative Standard Curve using ab108665

    Protocols

    References for Cortisol ELISA Kit (ab108665)

    ab108665 has not yet been referenced specifically in any publications.

    Product Wall

    We do not sell the Cortisol-HRP reagent separately from this kit.

    Abreviews
    Tested the kit in mouse. I was interested in seeing if
    this kit cross-reacts with corticosterone.

    I have tested this kit with different plasma samples of which I knew the concentration of corticosterone.
    Username

    Ms. Isabelle Barnola

    Verified customer

    Submitted Apr 02 2014

    excellent product

    Excellent Excellent 5/5 (Ease of Use)
    Abreviews
    very easy to use and accurate results in a few hours
    Username

    Abcam user community

    Verified customer

    Submitted Jan 29 2014


    I confirmed with the lab and unfortunately do not have good news:
    The heavily hemolyzed samples would have an great influence on the results. We do not know of any protocol trick to circumvent this and therefore recommend to collect new sampl...

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    Thank you for contacting us.

    The Cortisol ELISA kit is validated for use on Human serum and plasma samples. We have not validated the use of Mouse samples in this kit, however, plasma samples have been validated. As such, it should be ok to co...

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    Malheureusement non, nous n'avons pas de kit pour le dosage de l'ACTH ou du CRH spinal.

    Désolé de ne pas pouvoir vous aider davantage. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

    Merci de nous avoir contactés.

    Nous avons à notre catalogue le kit http://www.abcam.com/ab108665, Cortisol Human (competitive)ELISA Kit.


    Ce kit a été testé chez l'humain uniquement, il fonction...

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    Thank you for contacting us.  The Cortisol ELISA Kit ab108665 detects total Cortisol.  I hope this helps, please let me know if you need any additional information.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"