Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in serum and plasma.
A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where any Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.
|Components||1 x 96 tests|
|10X Washing Solution||1 x 50ml|
|Anti-Cortisol (IgG) Coated Microplate (12 x 8 wells)||1 unit|
|Cortisol Control||1 x 1ml|
|Cortisol Standard 0 – 0 ng/mL||1 x 1ml|
|Cortisol Standard 1 – 10 ng/mL||1 x 1ml|
|Cortisol Standard 2 – 50 ng/mL||1 x 1ml|
|Cortisol Standard 3 – 150 ng/mL||1 x 1ml|
|Cortisol Standard 4 – 500 ng/mL||1 x 1ml|
|Cortisol-HRP conjugate||1 x 21ml|
|Stop Solution||1 x 15ml|
|TMB Substrate Solution||1 x 15ml|
Our Abpromise guarantee covers the use of ab108665 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Competitive ELISA||Use at an assay dependent dilution.|
Cortisol measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.
Representative Standard Curve using ab108665